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MB Sample ID: SA204256
| Local Sample ID: | ASPC-G-1-r002 |
| Subject ID: | SU002207 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002207 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| ASPC-G-1-r002 | SA204256 | FL025057 | After inoculating cells for 24h in 10cm dishes, treating cells with gemcitabine with a final concentration of 50μM for 72h | Treatment |
Collection:
| Collection ID: | CO002200 |
| Collection Summary: | After 72 hours of drug treatment, the cells were washed twice with ice PBS and scraped in 80% ice methanol |
| Sample Type: | Tumor cells |
Treatment:
| Treatment ID: | TR002219 |
| Treatment Summary: | Cells were evenly divided into 3 groups of 6 discs each. 24h after cell inoculation, gemcitabine administration group was treated with gemcitabine with final concentration of 50μM for 72h, while the GI group was supplemented with an additional 200μM 3-isobutyl-1-methylxanthine (IBMX) in the culture medium. Meanwhile, the control group was given the same volume of DMSO. One plate of cells was taken from each group for counting, and the rest were collected for metabolite extraction |
Sample Preparation:
| Sampleprep ID: | SP002213 |
| Sampleprep Summary: | The cells were cultured as described above and fixed with 1 ml of 80% ice-cold menthol after being washed twice with ice-cold PBS. The cells were scraped from the plates, and 0.5-mm beads were added to process the cells by grinding and shaking. The supernatants were collected after centrifugation and deproteinized by mixing with 800μL acetonitrile on ice. Then, the supernatants collected and spun down under nitrogen at room temperature. The samples were resuspended in 100 μL of distilled H2O, and 5 μL was used for LC-TQ-MS-based metabolome assay. |
Combined analysis:
| Analysis ID | AN003474 | AN003475 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Waters Acquity HSS T3 (100 x2.1mm,1.8um) | Waters Acquity HSS T3 (100 x2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6495 QQQ | Agilent 6495 QQQ |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | counts | counts |
Chromatography:
| Chromatography ID: | CH002564 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity HSS T3 (100 x2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003235 |
| Analysis ID: | AN003474 |
| Instrument Name: | Agilent 6495 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Agilent MassHunter Workstation Data Acquisition Agilent MassHunter |
| Ion Mode: | POSITIVE |
| MS ID: | MS003236 |
| Analysis ID: | AN003475 |
| Instrument Name: | Agilent 6495 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Agilent MassHunter Workstation Data Acquisition Agilent MassHunter |
| Ion Mode: | NEGATIVE |
