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MB Sample ID: SA204781
| Local Sample ID: | 10pos1 |
| Subject ID: | SU002218 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 40.3 ± 3.9 |
| Human Nutrition: | Effervecent glutamine administration (12.5g, once a day for 30 days) |
| Human Inclusion Criteria: | Patients living with HIV from Public Service Health Care for Sexually Transmitted Diseases/AIDS Center of Maringá City, Paraná, Brazil, with same ART treatment for at least 1 year. |
| Human Exclusion Criteria: | Patients which were transferred, who abandoned treatment, and those who had not been evaluated for viral load and CD4+ T lymphocyte counts were excluded. |
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Subject:
| Subject ID: | SU002218 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 40.3 ± 3.9 |
| Human Nutrition: | Effervecent glutamine administration (12.5g, once a day for 30 days) |
| Human Inclusion Criteria: | Patients living with HIV from Public Service Health Care for Sexually Transmitted Diseases/AIDS Center of Maringá City, Paraná, Brazil, with same ART treatment for at least 1 year. |
| Human Exclusion Criteria: | Patients which were transferred, who abandoned treatment, and those who had not been evaluated for viral load and CD4+ T lymphocyte counts were excluded. |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 10pos1 | SA204781 | FL025119 | AT | Sample class |
Collection:
| Collection ID: | CO002211 |
| Collection Summary: | Serum samples were obtained from 12 HIV/AIDS patients before (day 0) and after glutamine (day 31) administration, and from healthy donors. |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002230 |
| Treatment Summary: | Glutamine supplementation (12.5g/day for 30 days) |
Sample Preparation:
| Sampleprep ID: | SP002224 |
| Sampleprep Summary: | Serum samples were carefully separated and aliquoted in duplicates (250 μL each one) into cryovials and stored at -80 °C. Samples (250 μL) were thawed on ice, mixed with 250 μL of deuterium oxide (D2O 99.9%, Cambridge Isotope Laboratories, Inc., Andover, USA) at room temperature, centrifuged at 12,000 × g for 2 min at 4 °C, and transferred into 5 mm NMR tubes |
| Processing Storage Conditions: | -80℃ |
Analysis:
| MB Sample ID: | SA204781 |
| Analysis ID: | AN003488 |
| Analysis Type: | NMR |
| Results File: | ST002133_AN003488_Results.txt |
| Units: | intensity |
NMR:
| NMR ID: | NM000236 |
| Analysis ID: | AN003488 |
| Instrument Name: | Avance 600 Bruker |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 600 MHz for 1H |
| NMR Probe: | inverse triple-core probe (TBI) |
| NMR Solvent: | H2O/D2O |
| NMR Tube Size: | 5 mm |
