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MB Sample ID: SA205245
| Local Sample ID: | 2.5uM_run1 |
| Subject ID: | SU002221 |
| Subject Type: | Synthetic sample |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002221 |
| Subject Type: | Synthetic sample |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 2.5uM_run1 | SA205245 | FL025172 | standard mix | factor |
Collection:
| Collection ID: | CO002214 |
| Collection Summary: | To improve CE peak shape and support electrophoretic focusing, a sample diluent consisting of 133 mM ammonium acetate, and 0.1% formic acid was prepared.13 This sample diluent was spiked with 1 µM 13C phenylalanine, 3 µM 13C6 arginine and 0.8 µM 13C methionine D3 as internal standards. Calibration mixtures were prepared from serial dilution of stock standard solutions using the spiked sample diluent in a 1:4 ratio. Each calibration standard was analyzed twice to yield calibration curves, calculate figures of merit, and perform metabolite quantification. |
| Sample Type: | Synthetic Mixture |
Treatment:
| Treatment ID: | TR002233 |
| Treatment Summary: | To improve CE peak shape and support electrophoretic focusing, a sample diluent consisting of 133 mM ammonium acetate, and 0.1% formic acid was prepared.13 This sample diluent was spiked with 1 µM 13C phenylalanine, 3 µM 13C6 arginine and 0.8 µM 13C methionine D3 as internal standards. Calibration mixtures were prepared from serial dilution of stock standard solutions using the spiked sample diluent in a 1:4 ratio. Each calibration standard was analyzed twice to yield calibration curves, calculate figures of merit, and perform metabolite quantification. |
Sample Preparation:
| Sampleprep ID: | SP002227 |
| Sampleprep Summary: | To improve CE peak shape and support electrophoretic focusing, a sample diluent consisting of 133 mM ammonium acetate, and 0.1% formic acid was prepared.13 This sample diluent was spiked with 1 µM 13C phenylalanine, 3 µM 13C6 arginine and 0.8 µM 13C methionine D3 as internal standards. Calibration mixtures were prepared from serial dilution of stock standard solutions using the spiked sample diluent in a 1:4 ratio. Each calibration standard was analyzed twice to yield calibration curves, calculate figures of merit, and perform metabolite quantification. |
Combined analysis:
| Analysis ID | AN003497 |
|---|---|
| Analysis type | MS |
| Chromatography type | CE |
| Chromatography system | ZipChip (908 Devices) |
| Column | HS chip |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Exactive Plus Orbitrap |
| Ion Mode | POSITIVE |
| Units | Micromolars |
Chromatography:
| Chromatography ID: | CH002583 |
| Instrument Name: | ZipChip (908 Devices) |
| Column Name: | HS chip |
| Chromatography Type: | CE |
MS:
| MS ID: | MS003257 |
| Analysis ID: | AN003497 |
| Instrument Name: | Thermo Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All experiments were performed in positive ionization mode in the 50-500 m/z range at a mass resolution setting of 17,500. The capillary temperature was set to 200 °C and the sheath gas flow rate was 2 psi. The automatic gain control (AGC) target value was set to 3E6 and the maximum injection time was 20 ms. Data were acquired using Xcalibur 3.0 (Thermo Scientific) and were imported to Skyline software14 for peak picking and integration. The peak picking procedure used the analyte accurate m/z and migration time. Peak areas obtained from Skyline were exported as spreadsheets for further analysis. Quantitation was performed with the analyte peak areas relative to the peak area of one of the three isotopically labeled internal standards (13C6 arginine, 13C methionine D3 and 13C phenylalanine) chosen based on migration time similarities. |
| Ion Mode: | POSITIVE |
