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MB Sample ID: SA205348
| Local Sample ID: | Gastrolobium_HILICpos_09 |
| Subject ID: | SU002223 |
| Subject Type: | Plant |
| Subject Species: | Gastrolobium bilobum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002223 |
| Subject Type: | Plant |
| Subject Species: | Gastrolobium bilobum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Gastrolobium_HILICpos_09 | SA205348 | FL025184 | Gastrolobium Leaf | Tissue Factor |
| Gastrolobium_HILICpos_09 | SA205348 | FL025184 | FE 100uM NaF | Treatment Factor |
Collection:
| Collection ID: | CO002216 |
| Collection Summary: | Gastrolobium bilobum plants were grown in the presence or absence of sodium fluoride and were also fed different isotopically labeled precursors. Different tissues were collected to determine tissue specific concentration of fluoroacetate |
| Sample Type: | Plant |
Treatment:
| Treatment ID: | TR002235 |
| Treatment Summary: | Different tissues were collected from plants and different chemical exposures were applied to different plants. |
Sample Preparation:
| Sampleprep ID: | SP002229 |
| Sampleprep Summary: | Samples were extracted using acetonitrile and MTBE in biphasic extraction to reduce the amount of lipids in sample. Aqueous phase was analyzed. |
Combined analysis:
| Analysis ID | AN003499 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Orbitrap Exploris 240 |
| Ion Mode | POSITIVE |
| Units | Peak Area |
Chromatography:
| Chromatography ID: | CH002585 |
| Chromatography Summary: | HILIC analysis |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS003259 |
| Analysis ID: | AN003499 |
| Instrument Name: | Thermo Orbitrap Exploris 240 |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Most data files contain only MS1 data, with iterative exclusion MS/MS collected on pooled samples. Data was processed in MSDIAL v. 4.8 |
| Ion Mode: | POSITIVE |
