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MB Sample ID: SA205434

Local Sample ID:Gastrolobium_HILICneg_60
Subject ID:SU002224
Subject Type:Plant
Subject Species:Gastrolobium bilobum

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Subject:

Subject ID:SU002224
Subject Type:Plant
Subject Species:Gastrolobium bilobum

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
Gastrolobium_HILICneg_60SA205434FL025211Pooled SampleTissue factor
Gastrolobium_HILICneg_60SA205434FL025211No TreatmentTreatment factor

Collection:

Collection ID:CO002217
Collection Summary:Plants were grown in presence or absence of sodium fluoride or given isotopically labeled precursors to test possible pathways towards fluoroacetate synthesis.
Sample Type:Plant

Treatment:

Treatment ID:TR002236
Treatment Summary:Plants were grown in presence or absence of sodium fluoride, or fed isotopically labeled chemicals. Different plant tissues were collected from these plants.

Sample Preparation:

Sampleprep ID:SP002230
Sampleprep Summary:Plant tissues were frozen, ground, and extracted with biphasic MTBE water and acetonitrile. The aqueous phase was collected for analysis.

Combined analysis:

Analysis ID AN003500
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column Waters Acquity BEH (50 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Orbitrap Exploris 240
Ion Mode NEGATIVE
Units Peak Area

Chromatography:

Chromatography ID:CH002586
Chromatography Summary:HILIC chromatography
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH (50 x 2.1mm,1.7um)
Chromatography Type:HILIC

MS:

MS ID:MS003260
Analysis ID:AN003500
Instrument Name:Thermo Orbitrap Exploris 240
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Most samples have only MS1 data. Pooled samples were analyzed with iterative exclusion MS/MS on pooled samples.
Ion Mode:NEGATIVE
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