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MB Sample ID: SA205734
| Local Sample ID: | OI1_D1_S2_LE |
| Subject ID: | SU002227 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002227 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| OI1_D1_S2_LE | SA205734 | FL025236 | D1 | Experiment day |
Collection:
| Collection ID: | CO002220 |
| Collection Summary: | At days 0, 1, 7, 14, and 21 cells were trypsinized, filtered through 100 μm pore strainers, and rinsed twice with PBS, and at least 1 × 10 6 (range 1–4.5 × 10 6) cells per pellet were collected for lipidomics. |
| Sample Type: | Stem cells |
Treatment:
| Treatment ID: | TR002239 |
| Treatment Summary: | hAMSCs were detached, at passage 7, from the flasks by trypsinization, counted in a Neubauer chamber and seeded at a density of 0.5 × 10 6 cells/flask. Cells were maintained under basal conditions until reaching ~100% confluence, then the basal culture medium was exchanged and supplemented with osteoinductive factors, specifically 10 mM β-glycerophosphate (β-GP, Sigma-Aldrich G9422), 50 µg/mL L-ascorbic acid (Sigma A0278), and 10 nM Dexa (ACROS Organics™ 230300010). |
Sample Preparation:
| Sampleprep ID: | SP002233 |
| Sampleprep Summary: | Cellular metabolites were extracted using the methanol-chloroform-water method and the resulting lipophilic extracts were dried under a nitrogen flow. Prior to NMR analysis, dried lipidic extracts were re-suspended in deuterated chloroform (99.8% deuterium, Eurisotop D307F) containing 0.03% tetramethylsilane (TMS), homogenised and transferred to 5 mm NMR tubes. |
| Extraction Method: | Methanol-chloroform-water method |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Deuterated chloroform (99.8% deuterium, Eurisotop D307F) containing 0.03% tetramethylsilane (TMS) |
Analysis:
| MB Sample ID: | SA205734 |
| Analysis ID: | AN003507 |
| Analysis Type: | NMR |
| Software Version: | Topspin 4.0.8 |
| Operator Name: | Daniela Bispo |
| Results File: | ST002142_AN003507_Results.txt |
| Units: | ppm |
NMR:
| NMR ID: | NM000237 |
| Analysis ID: | AN003507 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Field Frequency Lock: | CDCl3 |
| Spectrometer Frequency: | 500 MHz |
| NMR Probe: | TXI |
| NMR Solvent: | CDCl3 |
| NMR Tube Size: | 5 mm |
| Shimming Method: | Topshim |
| Receiver Gain: | 203 |
| Temperature: | 298 K |
| Number Of Scans: | 512 |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.3 s |
| Relaxation Delay: | 4 s |
| Spectral Width: | 7002.801 Hz |
| Num Data Points Acquired: | 32k |
| Zero Filling: | 65k |
| Baseline Correction Method: | Manual |
| Chemical Shift Ref Std: | TMS |
