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MB Sample ID: SA205820
| Local Sample ID: | MV23 |
| Subject ID: | SU002233 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl/6 |
| Age Or Age Range: | 20 weeks (at euthanasia) |
| Gender: | Female |
| Animal Animal Supplier: | Jackson Labratories |
| Animal Light Cycle: | Standard light dark cycle |
| Animal Feed: | PicoLab Rodent Diet 20, 20% protein |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002233 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl/6 |
| Age Or Age Range: | 20 weeks (at euthanasia) |
| Gender: | Female |
| Animal Animal Supplier: | Jackson Labratories |
| Animal Light Cycle: | Standard light dark cycle |
| Animal Feed: | PicoLab Rodent Diet 20, 20% protein |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| MV23 | SA205820 | FL025259 | F | Sex |
| MV23 | SA205820 | FL025259 | bone_marrow | Tissue |
Collection:
| Collection ID: | CO002226 |
| Collection Summary: | Samples were collected following mouse euthanasia. The humerus was obtained and bone marrow was flushed resulting in 3 groups: isolated bone, bone marrow, and whole bone (has intact marrow). |
| Sample Type: | Bone and bone marrow |
Treatment:
| Treatment ID: | TR002245 |
| Treatment Summary: | Mice were housed at Montana State University after being obtained from Jackson labs. During this time, mice were kept on a light/dark cycle and fed a standard chow diet ad libitum. Upon 20 weeks of age, mice were euthanized. No additional factors such as injury, diet, or exercise occurred. Mice were simply used to analyze differences in metabolism between musculoskeletal tissues. |
Sample Preparation:
| Sampleprep ID: | SP002239 |
| Sampleprep Summary: | Once euthanized, tissues were dissected and harvested. Samples underwent a methanol:acetone extraction and underwent rounds of centrifugation. Once metabolites were isolated, samples were dried via vacuum concentration and resuspended with acetonitrile:water for LC-MS analysis. |
Combined analysis:
| Analysis ID | AN003516 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 6538 |
| Column | Cogent Diamond Hydride HILIC (150 × 2.1 mm) |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 6538 QTOF |
| Ion Mode | POSITIVE |
| Units | Peak Intensiy |
Chromatography:
| Chromatography ID: | CH002596 |
| Chromatography Summary: | Samples were analyzed using an Agilent 1290 UPLC coupled to an Agilent 6538 QTOF. All samples were run in normal phase using a Cogent Diamond Hydride HILIC column (150 x 2.1 mm). Solvents: 0.1% formic acid in water (A), 0.1% formic acid in acetonitrile (B). Elution gradient: 100 to 25% solvent B over 17 minutes, 0 to 70% solvent A over 20 minutes, at 21 minutes, B increases from 25% to 100% till 25 minutes while A decreases from 70% to 0% till 25 minutes. The last 4 minutes act as a wash. Total run time = 25 minute method (per sample) |
| Instrument Name: | Agilent 6538 |
| Column Name: | Cogent Diamond Hydride HILIC (150 × 2.1 mm) |
| Flow Gradient: | 100 to 25% solvent B over 17 minutes, 0 to 70% solvent A over 20 minutes, at 21 minutes, B increases from 25% to 100% till 25 minutes while A decreases from 70% to 0% till 25 minutes. The last 4 minutes act as a wash. |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS003274 |
| Analysis ID: | AN003516 |
| Instrument Name: | Agilent 6538 QTOF |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
