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MB Sample ID: SA205928
Local Sample ID: | CSAR8 |
Subject ID: | SU002235 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Clostridioides difficile ATCC43255 |
Age Or Age Range: | 6 weeks |
Gender: | Male and female |
Animal Animal Supplier: | Massachusetts Host-Microbiome Center Gnotobiotic Resource |
Animal Housing: | Germfree |
Animal Water: | sterile, ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002235 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Clostridioides difficile ATCC43255 |
Age Or Age Range: | 6 weeks |
Gender: | Male and female |
Animal Animal Supplier: | Massachusetts Host-Microbiome Center Gnotobiotic Resource |
Animal Housing: | Germfree |
Animal Water: | sterile, ad libitum |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
CSAR8 | SA205928 | FL025270 | C. sardiniense monocolonized | Treatment |
Collection:
Collection ID: | CO002228 |
Collection Summary: | Cecal contents |
Sample Type: | Cecum |
Collection Method: | Snap freeze in cryovials on liquid nitrogen |
Storage Conditions: | -80℃ |
Additives: | None |
Treatment:
Treatment ID: | TR002247 |
Treatment Summary: | Gnotobiotic mice (baseline controls) or mono- or co-colonized with C. difficile, and the commensal P. bifermentans or C. sardiniense. |
Treatment Dose: | gavage with 1000 spores of ATCC43255 for C. difficile infection; 200uL of overnight culture for commensal species |
Sample Preparation:
Sampleprep ID: | SP002241 |
Sampleprep Summary: | Cecum was dissected from mice and contents immediately transferred into cryovials for snap freezing on liquid nitrogen. Materials stored at -80*C until shipment to Metabolon, on dry ice, for analyses. |
Processing Storage Conditions: | -80℃ |
Extract Storage: | -80℃ |
Combined analysis:
Analysis ID | AN003519 |
---|---|
Analysis type | MS |
Chromatography type | Unspecified |
Chromatography system | Unspecified |
Column | Unspecified |
MS Type | Unspecified |
MS instrument type | Unspecified |
MS instrument name | Metabolon |
Ion Mode | UNSPECIFIED |
Units | OriginalScale MS Units |
Chromatography:
Chromatography ID: | CH002598 |
Chromatography Summary: | None |
Instrument Name: | Unspecified |
Column Name: | Unspecified |
Chromatography Type: | Unspecified |
MS:
MS ID: | MS003277 |
Analysis ID: | AN003519 |
Instrument Name: | Metabolon |
Instrument Type: | Unspecified |
MS Type: | Unspecified |
MS Comments: | Metabolon global untargeted screen of cecal contents |
Ion Mode: | UNSPECIFIED |