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MB Sample ID: SA207607
Local Sample ID: | R_5 Gy 4 |
Subject ID: | SU002252 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002252 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
R_5 Gy 4 | SA207607 | FL025458 | 5Gy Irradiated | Treatment |
Collection:
Collection ID: | CO002245 |
Collection Summary: | Animals were acclimatized for 48 hrs in polypropylene cages. Urine samples were collected at 24 hrs post radiation exposure. |
Sample Type: | Urine |
Storage Conditions: | -80℃ |
Collection Tube Temp: | 4℃ |
Treatment:
Treatment ID: | TR002264 |
Treatment Summary: | Random categorization was done into three groups: control, 5 Gy (sub-lethal) and 7.5 Gy (lethal) irradiated (seven animals in each group). Mice were kept for 12 hrs light and dark cycle with room temperature (19–23°C) and humidity (45–65%) conditions properly maintained. Mice were exposed to total-body gamma radiation (field size; 35x35 cm) of 5 Gy and 7.5 Gy through Tele 60Co unit γ- irradiation facility (Bhabhatron II, Panacea Medical, Bangalore, India) with dose rate of 1 Gy/min. Control mice were sham irradiated. |
Treatment: | Radiation exposure |
Treatment Dose: | 5 Gy and 7.5 Gy Gamma-60Cobalt irradiation |
Sample Preparation:
Sampleprep ID: | SP002258 |
Sampleprep Summary: | 150 µl of urine was mixed with 375 µl of deuterated phosphate buffer solution (pH 7.2- 7.4) containing 0.5 mM TSP prepared in D2O. The solution was then transferred to 5 mm NMR tube for data acquisition |
Analysis:
MB Sample ID: | SA207607 |
Analysis ID: | AN003549 |
Analysis Type: | NMR |
Results File: | ST002166_AN003549_Results.txt |
Units: | Integral |
NMR:
NMR ID: | NM000239 |
Analysis ID: | AN003549 |
Instrument Name: | Bruker |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 600MHz |