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MB Sample ID: SA207612
| Local Sample ID: | R_7.5 Gy 2 |
| Subject ID: | SU002252 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002252 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| R_7.5 Gy 2 | SA207612 | FL025459 | 7.5Gy Irradiated | Treatment |
Collection:
| Collection ID: | CO002245 |
| Collection Summary: | Animals were acclimatized for 48 hrs in polypropylene cages. Urine samples were collected at 24 hrs post radiation exposure. |
| Sample Type: | Urine |
| Storage Conditions: | -80℃ |
| Collection Tube Temp: | 4℃ |
Treatment:
| Treatment ID: | TR002264 |
| Treatment Summary: | Random categorization was done into three groups: control, 5 Gy (sub-lethal) and 7.5 Gy (lethal) irradiated (seven animals in each group). Mice were kept for 12 hrs light and dark cycle with room temperature (19–23°C) and humidity (45–65%) conditions properly maintained. Mice were exposed to total-body gamma radiation (field size; 35x35 cm) of 5 Gy and 7.5 Gy through Tele 60Co unit γ- irradiation facility (Bhabhatron II, Panacea Medical, Bangalore, India) with dose rate of 1 Gy/min. Control mice were sham irradiated. |
| Treatment: | Radiation exposure |
| Treatment Dose: | 5 Gy and 7.5 Gy Gamma-60Cobalt irradiation |
Sample Preparation:
| Sampleprep ID: | SP002258 |
| Sampleprep Summary: | 150 µl of urine was mixed with 375 µl of deuterated phosphate buffer solution (pH 7.2- 7.4) containing 0.5 mM TSP prepared in D2O. The solution was then transferred to 5 mm NMR tube for data acquisition |
Analysis:
| MB Sample ID: | SA207612 |
| Analysis ID: | AN003549 |
| Analysis Type: | NMR |
| Results File: | ST002166_AN003549_Results.txt |
| Units: | Integral |
NMR:
| NMR ID: | NM000239 |
| Analysis ID: | AN003549 |
| Instrument Name: | Bruker |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 600MHz |
