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MB Sample ID: SA211352

Local Sample ID:MB-ES-121102-37_SARC
Subject ID:SU002288
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6 (WT); LSL-KrasG12D/+ - p48Cre/+; LSL-KrasG12/D+; LSL-Tpr53R172H/+; Pdx-1-Cre
Age Or Age Range:1.5 to 9 months
Animal Light Cycle:12h light/dark cycles (7.00 AM lights on)
Animal Feed:ad libitum
Animal Water:ad libitum
Animal Inclusion Criteria:Healthy animails
Species Group:C57BL/6 (WT); LSL-KrasG12D/+ - p48Cre/+; LSL-KrasG12/D+; LSL-Tpr53R172H/+; Pdx-1-Cre

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Subject:

Subject ID:SU002288
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6 (WT); LSL-KrasG12D/+ - p48Cre/+; LSL-KrasG12/D+; LSL-Tpr53R172H/+; Pdx-1-Cre
Age Or Age Range:1.5 to 9 months
Animal Light Cycle:12h light/dark cycles (7.00 AM lights on)
Animal Feed:ad libitum
Animal Water:ad libitum
Animal Inclusion Criteria:Healthy animails
Species Group:C57BL/6 (WT); LSL-KrasG12D/+ - p48Cre/+; LSL-KrasG12/D+; LSL-Tpr53R172H/+; Pdx-1-Cre

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
MB-ES-121102-37_SARCSA211352FL025771SarcomatoidTreatment_group

Collection:

Collection ID:CO002281
Collection Summary:Mice were sacrificed by cervical dislocation and pancreatic tissue rapidly excised and freeze-clamped using liquid nitrogen-cooled tongs.
Sample Type:Pancreas

Treatment:

Treatment ID:TR002300
Treatment Summary:The control group (N, normal tissue, n=7) comprised animals that did not develop lesions or tumors (C57BL/6 wt mice); the pancreatitis group (Pt, n=6) comprised C57BL/6 wt mice in which acute pancreatitis was induced by six hourly intraperitoneal 50 ug/kg caerulein (Sigma-Aldrich, Dorset, UK) injections; the low grade lesion group (LG, n=5) comprised 4-month old KC (LSL-KrasG12D/+ - p48Cre/+) mice with predominant LG PanIN lesions (mPanIN 1 and 1A); the high grade lesion group (HG, n=7) comprised 9-month old KC mice with predominant HG PanIN lesions (mPanIN 2 and 3); the spontaneous pancreatic ductal adenocarcinoma (PDA, n=6) and sarcomatoid (Sarc., n=9) groups corresponded to 3-6 month old KPC (LSL-KrasG12/D+;LSL-Tpr53R172H/+;Pdx-1-Cre) mice. Tumors were studied when they were less than 5 mm in diameter.

Sample Preparation:

Sampleprep ID:SP002294
Sampleprep Summary:Tissues (weights in the 60-120 mg range) were homogenized (ca. 200mg/mL) in 50 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), 1 mM EDTA (ethylenediaminetetraacetic acid), 0.7% sodium deoxy- cholate, 1% Nonidet P-40, 0.5 M lithium chloride, pH 7.6, using a Precellys 24 homogeniser (Stretton Scientific, Stretton, UK) and then extracted in methanol:chloroform:water.
Processing Storage Conditions:-80℃
Extraction Method:Biphasic method (methanol/ chloroform/ water)
Extract Storage:-80℃

Analysis:

MB Sample ID:SA211352
Analysis ID:AN003605
Laboratory Name:Metabolomics Ana M. Gil
Analysis Type:NMR
Software Version:Topspin 3.2
Results File:ST002202_AN003605_Results.txt
Units:ppmn

NMR:

NMR ID:NM000242
Analysis ID:AN003605
Instrument Name:Bruker 600 MHz NMR spectrometer
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
NMR Comments:1st subfolder contains raw spectra; 2nd subfolder contains manually processed spectra
Field Frequency Lock:H2O+D2O for 2H
Spectrometer Frequency:600MHz
NMR Solvent:D2O
NMR Tube Size:5mm
Shimming Method:Topshim
Receiver Gain:724.1
Chemical Shift Ref Cpd:3-(trimethylsilyl)-2,2’,3,3’-tetradeuteropropionic acid (TSP)
Temperature:298K
Number Of Scans:512
Acquisition Time:4.5 s
Relaxation Delay:12.5 s
Spectral Width:7.3 kHz
Num Data Points Acquired:32 k
Real Data Points:64 k
Zero Filling:64 k (and multiplied by a 0.3 Hz exponential function prior to Fourier transformation)
Baseline Correction Method:Manual
Chemical Shift Ref Std:3-(trimethylsilyl)-2,2’,3,3’-tetradeuteropropionic acid (TSP)
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