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MB Sample ID: SA217073
Local Sample ID: | 34984 |
Subject ID: | SU002340 |
Subject Type: | Mammal |
Subject Species: | Papio hamadryas |
Taxonomy ID: | 9557 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002340 |
Subject Type: | Mammal |
Subject Species: | Papio hamadryas |
Taxonomy ID: | 9557 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
34984 | SA217073 | FL026329 | SKELETAL MUSCLE | Sample type |
Collection:
Collection ID: | CO002333 |
Collection Summary: | The liver, skeletal muscle and plasma samples consisted of 32 samples each which were obtained from post-pubertal offspring (3-6.5y at sample collection) of MO mothers (n=19) and from control animals born to mothers fed a standard diet (CON, n=13). The datasets were generated from metabolic profiling of these tissues and plasma samples collected from 17 females in the age range of 3-6.5 years and 13 males in the same age range. All were analyzed using an untargeted EI-GC-MS approach as described above. |
Sample Type: | Liver |
Treatment:
Treatment ID: | TR002352 |
Treatment Summary: | We analyzed liver and muscle biopsies and plasma samples obtained from the post-pubertal offspring (3-6.5 years old at sample collection) of MO mothers (n=19, 10 females and 9 males) and from control animals born to mothers fed a standard diet (n=13, 6 females and 7 males). All offspring animals ate normal chow diet after weaning so differences between groups are due to the impact of the maternal intrauterine environment. |
Sample Preparation:
Sampleprep ID: | SP002346 |
Sampleprep Summary: | 15 μL of plasma, liver or skeletal muscle samples were subjected to sequential solvent extraction, once each with 1 mL of acetonitrile: isopropanol: water (3:3:2) and 500 μL of acetonitrile: water (1:1) mixtures at 4°C [14]. An internal standard, adonitol (2 μL from 10 mg/ml stock) was added to each aliquot prior to the extraction. The extracts were dried under vacuum at 4°C prior to chemical derivatization (silylation reactions). Blank tubes without samples, were treated similarly as sample tubes and added to account for background noise and other sources of contamination. Samples and blanks were sequentially derivatized with meth-oxyamine hydrochloride (MeOX) and 1% TMCS in N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) or 1% TMCS containing N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) as described elsewhere [15]. Briefly, the steps involved addition of 20 μL of MeOX (20 mg mL-1) in pyridine incu-bated at 55°C for 60 min followed by trimethylsilylation at 60°C for 60 min after adding 80 μL MTBSTFA. |
Combined analysis:
Analysis ID | AN003682 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Thermo Trace 1310 |
Column | Thermo Scientific Trace GOLD TG-5SIL-MS |
MS Type | EI |
MS instrument type | QTRAP |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | Normalized Peak Intensities |
Chromatography:
Chromatography ID: | CH002730 |
Instrument Name: | Thermo Trace 1310 |
Column Name: | Thermo Scientific Trace GOLD TG-5SIL-MS |
Chromatography Type: | GC |
MS:
MS ID: | MS003433 |
Analysis ID: | AN003682 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | QTRAP |
MS Type: | EI |
MS Comments: | Data acquisition and instrument control were carried out using Xcalibur 4.3 and Trace-Finder 4.1 softwares MS-DIAL |
Ion Mode: | POSITIVE |