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MB Sample ID: SA217145
Local Sample ID: | M37 |
Subject ID: | SU002342 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002342 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
M37 | SA217145 | FL026335 | M | Gender |
M37 | SA217145 | FL026335 | Obe | Group |
Collection:
Collection ID: | CO002335 |
Collection Summary: | This longitudinal cohort study is an analysis of serum samples collected from obese children before and after a 2-month weight reduction program that consisted of three visits to the hospital. |
Sample Type: | Blood (serum) |
Treatment:
Treatment ID: | TR002354 |
Treatment Summary: | Participants completed the questionnaires on general lifestyle (the time spent studying and using electronic devices, the duration and frequency of regular exercise, the presence of easily accessible locations to exercise, and their mode of transportation to school) and eating habits (meal duration, the consumption of late-night snacks, the consumption of breakfast, and the intake of sugar-sweetened beverages) and submitted them at the first hospital visit. |
Sample Preparation:
Sampleprep ID: | SP002348 |
Sampleprep Summary: | Frozen serum samples were thawed on ice. For preparation of the serum, a 50 µL sample was extracted using 1 mL of N2-degassed 1st extraction solution. Then, the samples were mixed for 10 min and centrifuged for 10 min at 18945 RCF and 4°C. The supernatant was dried for 6 hours at 45°C. The dried samples were re-extracted with 2nd extraction solution. Then, the extracted samples were redried for 8 hours under the same conditions used in the first extraction step. The dried samples were derivatized with methoxyamine at 30°C for 90 min and subsequently trimethylsilylated with a mixture of fatty acid methyl ester, which is used for the retention time index, in N-methyl-N-(trimethylsilyl)-trifluoroacetamide at 70°C for 45 min. |
Combined analysis:
Analysis ID | AN003685 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890B |
Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Leco Pegasus HT TOF |
Ion Mode | NEGATIVE |
Units | intensity |
Chromatography:
Chromatography ID: | CH002732 |
Instrument Name: | Agilent 7890B |
Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
Chromatography Type: | GC |
MS:
MS ID: | MS003436 |
Analysis ID: | AN003685 |
Instrument Name: | Leco Pegasus HT TOF |
Instrument Type: | GC-TOF |
MS Type: | EI |
MS Comments: | The LECO Corporation ChromaTOF®-GC software (v4.72) was used for data acquisition and extraction. This included computation of baseline (offset of 1; above the noise) and automatic entering the number of data points for smoothing. The software's Statistical Compare was used to align peaks. The MS spectral matching was performed using the NIST and Wiley9 libraries. Also, retention index method was used for identification. |
Ion Mode: | NEGATIVE |