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MB Sample ID: SA220015
Local Sample ID: | xeno_B_EA_A_32b_1_2 |
Subject ID: | SU002376 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | CBA nude (N:NIH(S)II-nu/nu) |
Age Or Age Range: | 6 to 7-weeks old |
Gender: | Female |
Animal Animal Supplier: | i3S Animal Facility (Porto, Portugal) |
Animal Housing: | ICBAS-UP Rodent Animal House Facility (Porto, Portugal) |
Animal Light Cycle: | 12h light/dark cycles (7.00 AM lights on) |
Animal Feed: | ad libitum |
Animal Water: | ad libitum |
Animal Inclusion Criteria: | Healthy animails |
Species Group: | CBA nude (N:NIH(S)II-nu/nu) |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002376 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | CBA nude (N:NIH(S)II-nu/nu) |
Age Or Age Range: | 6 to 7-weeks old |
Gender: | Female |
Animal Animal Supplier: | i3S Animal Facility (Porto, Portugal) |
Animal Housing: | ICBAS-UP Rodent Animal House Facility (Porto, Portugal) |
Animal Light Cycle: | 12h light/dark cycles (7.00 AM lights on) |
Animal Feed: | ad libitum |
Animal Water: | ad libitum |
Animal Inclusion Criteria: | Healthy animails |
Species Group: | CBA nude (N:NIH(S)II-nu/nu) |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
xeno_B_EA_A_32b_1_2 | SA220015 | FL027446 | Cisplatin-treated | Treatment_group |
Collection:
Collection ID: | CO002369 |
Collection Summary: | At day 39 post-implantation of MDA-MB-231 cells (25G needle, 5E6 cells in 150 microliters of PBS) in the left flank of mice, and, at day 9 post-treatment with metal-based drugs, the mice were sacrificed with isoflurane, organs were excised, snap-frozen and stored at -80 ºC. |
Sample Type: | Brain |
Collection Duration: | Inferior to one minute |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002388 |
Treatment Summary: | At day 25 post-implantation, 8 animals were randomly chosen into three groups to receive the treatment with either (i) vehicle (phosphate-buffered saline, PBS) - controls, (ii) cDDP (2 mg/kg/day), or (iii) Pd2Spm (5 mg/kg/day), via intraperitoneal injection, during five consecutive days. Two control animals were excluded from the study since they developed ulcerated tumors (at day 28 post-implantation) and needed to be prematurely euthanized. |
Treatment Route: | Intraperitoneal injection |
Treatment Dosevolume: | 500 microliters |
Treatment Vehicle: | PBS (phosphate-buffered saline solution) |
Sample Preparation:
Sampleprep ID: | SP002382 |
Sampleprep Summary: | The frontal cortex of each mice brain was mechanically grounded in liquid nitrogen and samples were extracted using the biphasic methanol/ chloroform/ water (2:2:1) method. Aqueous phase was recovered and dried. Previously to NMR acquisition, aqueous extracts were suspended in 650 µL of 100 mM sodium phosphate buffer (pH 7.4, in D2O containing 0.25% 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid (TSP) for chemical shift referencing). Samples were then homogenized and transferred into 5mm NMR tubes. |
Processing Storage Conditions: | -80℃ |
Extraction Method: | Biphasic method (methanol/ chloroform/ water) |
Extract Storage: | -80℃ |
Analysis:
MB Sample ID: | SA220015 |
Analysis ID: | AN003742 |
Laboratory Name: | Metabolomics Ana M. Gil |
Analysis Type: | NMR |
Acquisition Date: | October 2021 |
Software Version: | Topspin 3.2 |
Results File: | ST002290_AN003742_Results.txt |
Units: | ppm |
NMR:
NMR ID: | NM000249 |
Analysis ID: | AN003742 |
Instrument Name: | Avance III TM HD 500MHz |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
NMR Comments: | 1st subfolder contains raw spectra; 2nd subfolder contains manually processed spectra |
Field Frequency Lock: | Deuterium |
Spectrometer Frequency: | 500MHz |
NMR Probe: | TXI |
NMR Solvent: | D2O |
NMR Tube Size: | 5mm |
Shimming Method: | Topshim |
Receiver Gain: | 203 |
Temperature: | 298K |
Number Of Scans: | 512 |
Acquisition Time: | 2.34s |
Relaxation Delay: | 2s |
Spectral Width: | 7002.801 |
Zero Filling: | 64k |
Baseline Correction Method: | Manual |
Chemical Shift Ref Std: | TSP (3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid) |
NMR Results File: | BrainAE_NMR_data.txt UNITS:ppm |