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MB Sample ID: SA220017
| Local Sample ID: | xeno_B_EA_A_14_1_2 |
| Subject ID: | SU002376 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | CBA nude (N:NIH(S)II-nu/nu) |
| Age Or Age Range: | 6 to 7-weeks old |
| Gender: | Female |
| Animal Animal Supplier: | i3S Animal Facility (Porto, Portugal) |
| Animal Housing: | ICBAS-UP Rodent Animal House Facility (Porto, Portugal) |
| Animal Light Cycle: | 12h light/dark cycles (7.00 AM lights on) |
| Animal Feed: | ad libitum |
| Animal Water: | ad libitum |
| Animal Inclusion Criteria: | Healthy animails |
| Species Group: | CBA nude (N:NIH(S)II-nu/nu) |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002376 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | CBA nude (N:NIH(S)II-nu/nu) |
| Age Or Age Range: | 6 to 7-weeks old |
| Gender: | Female |
| Animal Animal Supplier: | i3S Animal Facility (Porto, Portugal) |
| Animal Housing: | ICBAS-UP Rodent Animal House Facility (Porto, Portugal) |
| Animal Light Cycle: | 12h light/dark cycles (7.00 AM lights on) |
| Animal Feed: | ad libitum |
| Animal Water: | ad libitum |
| Animal Inclusion Criteria: | Healthy animails |
| Species Group: | CBA nude (N:NIH(S)II-nu/nu) |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| xeno_B_EA_A_14_1_2 | SA220017 | FL027446 | Cisplatin-treated | Treatment_group |
Collection:
| Collection ID: | CO002369 |
| Collection Summary: | At day 39 post-implantation of MDA-MB-231 cells (25G needle, 5E6 cells in 150 microliters of PBS) in the left flank of mice, and, at day 9 post-treatment with metal-based drugs, the mice were sacrificed with isoflurane, organs were excised, snap-frozen and stored at -80 ºC. |
| Sample Type: | Brain |
| Collection Duration: | Inferior to one minute |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002388 |
| Treatment Summary: | At day 25 post-implantation, 8 animals were randomly chosen into three groups to receive the treatment with either (i) vehicle (phosphate-buffered saline, PBS) - controls, (ii) cDDP (2 mg/kg/day), or (iii) Pd2Spm (5 mg/kg/day), via intraperitoneal injection, during five consecutive days. Two control animals were excluded from the study since they developed ulcerated tumors (at day 28 post-implantation) and needed to be prematurely euthanized. |
| Treatment Route: | Intraperitoneal injection |
| Treatment Dosevolume: | 500 microliters |
| Treatment Vehicle: | PBS (phosphate-buffered saline solution) |
Sample Preparation:
| Sampleprep ID: | SP002382 |
| Sampleprep Summary: | The frontal cortex of each mice brain was mechanically grounded in liquid nitrogen and samples were extracted using the biphasic methanol/ chloroform/ water (2:2:1) method. Aqueous phase was recovered and dried. Previously to NMR acquisition, aqueous extracts were suspended in 650 µL of 100 mM sodium phosphate buffer (pH 7.4, in D2O containing 0.25% 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid (TSP) for chemical shift referencing). Samples were then homogenized and transferred into 5mm NMR tubes. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Biphasic method (methanol/ chloroform/ water) |
| Extract Storage: | -80℃ |
Analysis:
| MB Sample ID: | SA220017 |
| Analysis ID: | AN003742 |
| Laboratory Name: | Metabolomics Ana M. Gil |
| Analysis Type: | NMR |
| Acquisition Date: | October 2021 |
| Software Version: | Topspin 3.2 |
| Results File: | ST002290_AN003742_Results.txt |
| Units: | ppm |
NMR:
| NMR ID: | NM000249 |
| Analysis ID: | AN003742 |
| Instrument Name: | Avance III TM HD 500MHz |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| NMR Comments: | 1st subfolder contains raw spectra; 2nd subfolder contains manually processed spectra |
| Field Frequency Lock: | Deuterium |
| Spectrometer Frequency: | 500MHz |
| NMR Probe: | TXI |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm |
| Shimming Method: | Topshim |
| Receiver Gain: | 203 |
| Temperature: | 298K |
| Number Of Scans: | 512 |
| Acquisition Time: | 2.34s |
| Relaxation Delay: | 2s |
| Spectral Width: | 7002.801 |
| Zero Filling: | 64k |
| Baseline Correction Method: | Manual |
| Chemical Shift Ref Std: | TSP (3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid) |
| NMR Results File: | BrainAE_NMR_data.txt UNITS:ppm |
