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MB Sample ID: SA227042
| Local Sample ID: | 35 |
| Subject ID: | SU002398 |
| Subject Type: | Bird |
| Subject Species: | Gallus gallus |
| Taxonomy ID: | 9031 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002398 |
| Subject Type: | Bird |
| Subject Species: | Gallus gallus |
| Taxonomy ID: | 9031 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 35 | SA227042 | FL027569 | Phage | Group |
| 35 | SA227042 | FL027569 | Week 4 | Moment |
Collection:
| Collection ID: | CO002391 |
| Collection Summary: | serum samples were collected and immediately snap frozen for metabolomic analysis at the end of the experiment |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR002410 |
| Treatment Summary: | Half of the animals (25/50) in each experimental group (control and Φ-treated groups) were challenged with Salmonella Enteritidis (105 CFU/bird) at 24 h after placing. During the first 21 days of the chicken rearing period, Φ-treated group received 0.1% encapsulated Salmonella-phage (108 PFU/g) with the starter diet feed, and the control group did not receive any phage. |
Sample Preparation:
| Sampleprep ID: | SP002404 |
| Sampleprep Summary: | serum samples were lyophilized and homogenated. Then, 10 mg of the sample were mixed with 0.75mL of cold 75% (v/v) methanol, 0.1% (v/v) formic acid, spiked with 10μg/ml formononetin as internal standard. Then, the mix were shaked for 40’ at 20Hz using a Mixer Mill 300 (Qiagen) and centrifuged at 20,000 xg for 15 min at 4°C. The suspension (600μL) was transferred to a new 2-mL conical tube. |
Combined analysis:
| Analysis ID | AN003777 | AN003778 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Phenomenex Luna (100 x 2.1mm,2.5um) | Phenomenex Luna (100 x 2.1mm,2.5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | m/z ratio | m/z ratio |
Chromatography:
| Chromatography ID: | CH002793 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Phenomenex Luna (100 x 2.1mm,2.5um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003520 |
| Analysis ID: | AN003777 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | For the untargeted metabolomic analysis, Compound Discoverer software(Thermofisher Scientific) was used to identify the peaks, peak filtration, and peak alignment, and performed chromatogram alignment, peak picking, and public database (e.g., ChemSpider, KEGG, Metabolika) querying based on accurate masses(m/z). |
| Ion Mode: | POSITIVE |
| MS ID: | MS003521 |
| Analysis ID: | AN003778 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | For the untargeted metabolomic analysis, Compound Discoverer software(Thermofisher Scientific) was used to identify the peaks, peak filtration, and peak alignment, and performed chromatogram alignment, peak picking, and public database (e.g., ChemSpider, KEGG, Metabolika) querying based on accurate masses(m/z). |
| Ion Mode: | NEGATIVE |
