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MB Sample ID: SA235056
| Local Sample ID: | 326 |
| Subject ID: | SU002428 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 8-32-week-old |
| Weight Or Weight Range: | 25-50 |
| Gender: | Male |
| Animal Animal Supplier: | JAX |
| Animal Housing: | 5/cage |
| Animal Light Cycle: | 12h dark/12 h light |
| Animal Feed: | Special diets; see group labeling |
| Animal Water: | Ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002428 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 8-32-week-old |
| Weight Or Weight Range: | 25-50 |
| Gender: | Male |
| Animal Animal Supplier: | JAX |
| Animal Housing: | 5/cage |
| Animal Light Cycle: | 12h dark/12 h light |
| Animal Feed: | Special diets; see group labeling |
| Animal Water: | Ad libitum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 326 | SA235056 | FL029172 | Serine/glycine-free high fat diet + vehicle | Group |
Collection:
| Collection ID: | CO002421 |
| Collection Summary: | Liver samples were collected using Wollenberger clamps pre-cooled to the temperature of liquid nitrogen and stored at -80 C until analysis. |
| Sample Type: | Liver |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002440 |
| Treatment Summary: | Animals were subjected to 4 different conditions for a period of 24 weeks: 1) low fat diet + vehicle, 2) high fat diet + vehicle, 3) serine/glycine-free high fat diet + vehicle, and 4) serine/glycine-free high fat diet + myriocin. |
Sample Preparation:
| Sampleprep ID: | SP002434 |
| Sampleprep Summary: | Samples were extracted with 400 ul of methanol (-20 C), 100 ul of water (ice-cold), 400 saline (ice-cold), and 1000 ul of chloroform (-20 C). |
| Processing Storage Conditions: | Room temperature |
| Extract Storage: | 4℃ |
Combined analysis:
| Analysis ID | AN003822 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Ion trap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | normalized ion count/mg protein |
Chromatography:
| Chromatography ID: | CH002828 |
| Chromatography Summary: | 30% to 43% B from 3-8 min, then from 43% to 50% B from 8-9 min, then 50-90% B from 9-18 min, then 90-99% B from 18-26 min, then held at 99% B from 26-30 min, before returning to 30% B in 6 min and held for a further 4 min |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
| Column Temperature: | 40 |
| Flow Rate: | 0.2 ml/min |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003564 |
| Analysis ID: | AN003822 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Ion trap |
| MS Type: | ESI |
| MS Comments: | El-MAVEN |
| Ion Mode: | POSITIVE |
