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MB Sample ID: SA235264
| Local Sample ID: | 337 |
| Subject ID: | SU002432 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 8-32-week-old |
| Weight Or Weight Range: | 25-50 |
| Gender: | Male |
| Animal Animal Supplier: | JAX |
| Animal Housing: | 5/cage |
| Animal Light Cycle: | 12h dark/12 h light |
| Animal Feed: | Special diets; see group labeling |
| Animal Water: | Ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002432 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 8-32-week-old |
| Weight Or Weight Range: | 25-50 |
| Gender: | Male |
| Animal Animal Supplier: | JAX |
| Animal Housing: | 5/cage |
| Animal Light Cycle: | 12h dark/12 h light |
| Animal Feed: | Special diets; see group labeling |
| Animal Water: | Ad libitum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 337 | SA235264 | FL029185 | Serine/glycine-free high fat diet + myriocin | Group |
Collection:
| Collection ID: | CO002425 |
| Collection Summary: | Paw skin samples were collected using Wollenberger clamps pre-cooled to the temperature of liquid nitrogen and stored at -80 C until analysis. |
| Sample Type: | Paw skin |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002444 |
| Treatment Summary: | Animals were subjected to 4 different conditions for a period of 24 weeks: 1) low fat diet, 2) high fat diet, 3) serine/glycine-free high fat diet, and 4) serine/glycine-free high fat diet with myriocin. |
Sample Preparation:
| Sampleprep ID: | SP002438 |
| Sampleprep Summary: | Samples were extracted with 400 ul of methanol (-20 C), 100 ul of water (ice-cold), 400 saline (ice-cold), and 1000 ul of chloroform (-20 C). |
| Processing Storage Conditions: | Room temperature |
| Extract Storage: | 4℃ |
Combined analysis:
| Analysis ID | AN003827 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 6460C |
| Column | Spectra 3 μm C8SR 150 × 3 mm inner diameter, Peeke Scientific |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6460 QQQ |
| Ion Mode | POSITIVE |
| Units | normalized ion count/mg protein |
Chromatography:
| Chromatography ID: | CH002832 |
| Chromatography Summary: | The gradient elution program consisted of the following profile: 0 min, 82% B; 3 min, 82% B; 4 min, 90% B, 18 min, 99% B; 25 min, 99%, 27 min, 82% B, 30 min, 82% B. |
| Instrument Name: | Agilent 6460C |
| Column Name: | Spectra 3 μm C8SR 150 × 3 mm inner diameter, Peeke Scientific |
| Column Temperature: | 40 |
| Flow Rate: | 0.5 ml/min |
| Solvent A: | 100% water; 0.2% formic acid; 2 mM ammonium formate |
| Solvent B: | 100% methanol; 0.2% formic acid; 1 mM ammonium formate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003569 |
| Analysis ID: | AN003827 |
| Instrument Name: | Agilent 6460 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Mass Hunter |
| Ion Mode: | POSITIVE |
