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MB Sample ID: SA237988
| Local Sample ID: | PBS-3C-N |
| Subject ID: | SU002473 |
| Subject Type: | Cultured cells |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002473 |
| Subject Type: | Cultured cells |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| PBS-3C-N | SA237988 | FL029575 | Control | Treatment |
Collection:
| Collection ID: | CO002466 |
| Collection Summary: | RBL-2H3 cells were incubated with 200 ng/mL of IgE for 18 h, followed by co-stimulation with 500 ng/mL DNP-BSA for the Alg group, polyphenolic substances (curcumin or EGCG) and 500 ng/mL DNP-BSA for the intervention group, and the same volume of PBS solution for the PBS group. The cell samples were collected at two different time points, 1 h and 3 h after the effect, respectively. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR002485 |
| Treatment Summary: | RBL-2H3 cells were incubated with 200 ng/mL of IgE for 18 h, followed by co-stimulation with 500 ng/mL DNP-BSA for the Alg group, polyphenolic substances (curcumin or EGCG) and 500 ng/mL DNP-BSA for the intervention group, and the same volume of PBS solution for the PBS group. |
Sample Preparation:
| Sampleprep ID: | SP002479 |
| Sampleprep Summary: | The MeOH/MTBE/H2O system was used for the extraction of lipid metabolites from cell samples. Cell culture dishes stored at -80 °C were first removed on ice and 1 mL of methanolic solution containing internal standards was added, consisting of 1 μg/mL FFA (C16:0)-d3 and FFA (C18:0)-d3, 0.87 μg/mL LPC (19:0) and SM (d18:1/12:0), 1.12 μg/mL PC (38: 0), 0.92 μg/mL PE (30:0), 0.67 μg/mL TG (45:0), and 0.47 μg/mL Cer (d18:1/17:0). The cells were transferred to 5 mL Eppendorf centrifuge tubes by scraping with a cell scraper, adding 2.5 mL of MTBE and vortexing for 30 s. The cells were further shaken for 30 min at 10 °C and 1000 rpm using a thermostatic mixer. 750 μL of ultrapure water was added, vortexed for 60 s and allowed to stand for 5 min, and then centrifuged at 12,000 g for 10 min at 6 °C. After separation of the two phases, 400 μL of the hydrophobic phase was collected and dried in a cryogenic vacuum centrifuge concentrator, and the resulting lyophilized sample was stored at -80 °C. |
Combined analysis:
| Analysis ID | AN003883 | AN003884 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH002877 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002878 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003623 |
| Analysis ID: | AN003883 |
| Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Xcalibular |
| Ion Mode: | POSITIVE |
| MS ID: | MS003624 |
| Analysis ID: | AN003884 |
| Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Xcalibular |
| Ion Mode: | NEGATIVE |
