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MB Sample ID: SA243491
| Local Sample ID: | chdwb-urine009 |
| Subject ID: | SU002525 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 40-69 |
| Weight Or Weight Range: | 100-350 lbs |
| Height Or Height Range: | 58-78 inches |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002525 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 40-69 |
| Weight Or Weight Range: | 100-350 lbs |
| Height Or Height Range: | 58-78 inches |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| chdwb-urine009 | SA243491 | FL030504 | 7.257308358 | S-S PyC2-Gly (uM) |
Collection:
| Collection ID: | CO002518 |
| Collection Summary: | Samples were collected using standard operating procedures in the Center for Health Discovery and Well Being (CHDWB) cohort of healthy adults. The CHDWB enrolled 763 participants starting in 2008 with approval from the Emory Institutional Review Board (IRB approval No. 00007243). The original study included both genders and included individuals self-identifying as white, black, Hispanic and Asian. The present study selected available archival samples from 143 individuals without known disease. Urine samples included one sample per individual that was collected at either baseline, 6 months, or 12 months. Urine samples were stored at -80 ºC prior to analysis |
| Sample Type: | Urine |
Treatment:
| Treatment ID: | TR002537 |
| Treatment Summary: | This study used archival samples from an observational study. No treatment was performed. |
Sample Preparation:
| Sampleprep ID: | SP002531 |
| Sampleprep Summary: | Samples were prepared following the Emory University Clinical Biomarkers Laboratory SOP for high resolution metabolomics sample preparation. Urine was thawed from -80 °C on ice and vortexed to homogenize. 50 μL urine was transferred to a clean microcentrifuge tube and treated with with 100 μL LC-MS grade acetonitrile containing 2.5 μL internal standard solution. Urine was equilibrated on ice for 30 min. Precipitated proteins were removed by centrifuge (16100 x g at 4 °C for 10 min). 100 μL supernatant was removed and added to an autosampler vial. Samples were maintained at 4 °C until analysis. Samples were prepared alongside NIST SRM 1950 and q3June2014 reference standards. |
| Sampleprep Protocol Filename: | EmoryUniversity_HRM_QEHF-MassSpec_092017_v1.pdf |
Combined analysis:
| Analysis ID | AN003969 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters XBridge BEH Amide (50 x 2.1mm,2.5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH002935 |
| Methods Filename: | EmoryUniversity_HRM-QEHF_chromatography_5min_092017_v1.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters XBridge BEH Amide (50 x 2.1mm,2.5um) |
| Column Temperature: | 60 |
| Flow Gradient: | A= water, B= acetontrile, C= 2% formic acid in water; 22.5% A, 75% B, 2.5% C hold for 1.5 min, linear gradient to 77.5% A, 20% B, 2.5% C at 4 min, hold for 1 min |
| Flow Rate: | 0.35 mL/min for 1.5 min; linear increase to 0.4 mL/min at 4 min, hold for 1 min |
| Solvent A: | 100% water |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS003703 |
| Analysis ID: | AN003969 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Raw analysis files from urine analyses were converted to .mzXML using ProteoWizard v3. Data extraction was performed using apLCMS v6.3.3 and xMSanalyzer v2.0.8, generating m/z features consisting of an m/z, retention time (RT) and ion intensity. Feature and sample filtering retained features with a median CV of 50% or less, with a minimum mean Pearson correlation coefficient of 0.7 between technical replicates, and which were detected in at least 30% of samples. Technical replicates were median summarized. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | EmoryUniversity_HRM_QEHF-MassSpec_092017_v1.pdf |
