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MB Sample ID: SA246476
| Local Sample ID: | Batch1_SCH2 |
| Subject ID: | SU002553 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002553 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Batch1_SCH2 | SA246476 | FL030903 | 2.5 | diluted factor |
Collection:
| Collection ID: | CO002546 |
| Collection Summary: | Serum samples from conventional and GF mice were collected and stored at -80°C. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR002565 |
| Treatment Summary: | Thawed on ice, 25 µL of serum sample, was mixed with 100 µL extraction solvent of ice-cold methanol containing indole-3-acetic acid-d4 and kynurenic acid-d5. Mixture was vortexed and incubated at −20 °C for 30 min. Following centrifugation at 12,000 × g for 15 min at 4℃, 90 µL supernatant was collected and subsequently evaporated to dryness (Thermo Scientific, Waltham, MA) and dissolved in 45 µL of 10% acetonitrile containing 1 µM chlorpropamide. After centrifugation at 12,000 × g for 15 min at 4℃, supernatants were transferred to autosampler vials for LC-MS analysis. |
Sample Preparation:
| Sampleprep ID: | SP002559 |
| Sampleprep Summary: | GF mice were reared in standard, sterile (autoclaved cages containing sterile (autoclaved) bedding inside a hermetically sealed isolator with HEPA-filtered airflow and maintained on sterile (autoclaved) water and sterile (autoclaved) food (LabDiet® 5013, Land O’Lakes) ad libitum. One of two groups of conventional C57BL/6J mice with ad libitum access to a standard laboratory chow were switched onto a semi-purified diet (AIN-93G) while the second remained on standard chow. After one week, portal vein blood was collected. |
Combined analysis:
| Analysis ID | AN004018 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu 20AD |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple TOF |
| MS instrument name | ABI Sciex 5600 TripleTOF |
| Ion Mode | POSITIVE |
| Units | uM |
Chromatography:
| Chromatography ID: | CH002968 |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 55 |
| Flow Gradient: | The initial condition were 97% A and 3 % B, increasing to 45% B at 10 min, 75% B at 12 min where it was held at 75% B until 17.5 min before returning to the initial conditions. |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | Water with 0.1% formic acid |
| Solvent B: | Acetonitrile with 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003765 |
| Analysis ID: | AN004018 |
| Instrument Name: | ABI Sciex 5600 TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| MS Comments: | Peakview was used for data analysis. |
| Ion Mode: | POSITIVE |
