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MB Sample ID: SA247485
| Local Sample ID: | owl-1923-008 |
| Subject ID: | SU002558 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002558 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| owl-1923-008 | SA247485 | FL031112 | M | Gender |
| owl-1923-008 | SA247485 | FL031112 | CTL | Group |
Collection:
| Collection ID: | CO002551 |
| Collection Summary: | Samples were collected from the Biodonostia Health Research Institute. The inclusion criteria were asymptomatic individuals with intermediate risk. That is, individuals from 50 to 69 years that do not have clinical symptoms, and patients that do not have any family history or other factor associated with colorectal cancer. The study was conducted according to the clinical and ethical principals of the Spanish Government and the Declaration of Helsinki and it was approved by the Ethics Committee for Clinical Research of Euskadi (PI2013073). Informed consent was obtained from each individual. Patients and control individuals self-collected a fecal sample from one bowel movement without specific diet or medication restrictions at home the week before a colonoscopy was performed. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR002570 |
| Treatment Summary: | The fecal samples was delivered to the hospital and brought to the laboratory in less than 4 hours, split in aliquots and immediately frozen at -80 °C. one aliquot of each sample was employed for fecal occult blood (FOB) measurement. The remaining volume of each of the FOB extract was frozen at -80 °C until its use for metabolomics analysis. |
Sample Preparation:
| Sampleprep ID: | SP002564 |
| Sampleprep Summary: | SP.pdf contains all the detailed information related to sample preparation. |
| Sampleprep Protocol Filename: | SP.pdf |
Combined analysis:
| Analysis ID | AN004024 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity UPLC BEH C18 (100 x 1.0 mm, 1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters QTOF Premier |
| Ion Mode | POSITIVE |
| Units | normalized peak area |
Chromatography:
| Chromatography ID: | CH002974 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity UPLC BEH C18 (100 x 1.0 mm, 1.7um) |
| Column Temperature: | - |
| Flow Gradient: | A 10 minutes linear gradient increasing from 40% to 100% of B was used. After 5 minutes at 100% B, the mobile phase was reset to the initial conditions and maintained for 2 minutes. Total run 17 minutes. |
| Flow Rate: | 0.15 mL/min |
| Solvent A: | 40% water/60% acetonitrile; 10mM ammonium formate |
| Solvent B: | 25% acetonitrile/75% isopropanol; 10mM ammonium formate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003771 |
| Analysis ID: | AN004024 |
| Instrument Name: | Waters QTOF Premier |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The mass spectra data were acquired in positive ionization mode with capillary and cone voltages of 2000 and 35 V, respectively. The desolvation gas was set to 1000 L/h at a temperature of 400 °C. The cone gas was set to 30 L/h, and the source temperature was set to 120 °C. The MS detector operated in centroid acquisition mode for a m/z range of 50-1200, using an accumulation time of 0.5 s per spectrum. All spectra were mass corrected in real time by reference to leucine enkephaline, infused at 10 µL/min through an independent reference electrospray, sampled every 10 s. |
| Ion Mode: | POSITIVE |
