Return to study ST002481 main page
MB Sample ID: SA248260
| Local Sample ID: | 3_ICZ_UroA_120_1 |
| Subject ID: | SU002571 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002571 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 3_ICZ_UroA_120_1 | SA248260 | FL031206 | 120 | Time (min) |
Collection:
| Collection ID: | CO002564 |
| Collection Summary: | 40 µg microsomes isolated from Hepa 1 cells treated with 5 nM TCDD for 24 h, or lung microsomes (100 µg) from mice on the semi-purified diet, were incubated with various compounds and incubation times as indicated. The reactions were carried out at 37°C in an incubator in the presence of NADPH regeneration system and terminated by addition of 800 µL ice cold 100% methanol (v/v) containing 1 µM chlorpropamide as internal standard. |
| Sample Type: | Hepa 1 cells |
Treatment:
| Treatment ID: | TR002583 |
| Treatment Summary: | Then mixtures were kept in -20°C for 30 mins followed by centrifugation at 12,000 × g for 20 min at 4°C. The supernatants were dried in a under vacuum and reconstituted in 60 μL 50% methanol (v/v). After centrifugation, supernatants were transferred to autosampler vials for LC-MS/MS analysis. |
Sample Preparation:
| Sampleprep ID: | SP002577 |
| Sampleprep Summary: | 40 µg microsomes isolated from Hepa 1 cells treated with 5 nM TCDD for 24 h, or lung microsomes (100 µg) from mice on the semi-purified diet, were incubated with various compounds and incubation times as indicated. The reactions were carried out at 37°C in an incubator in the presence of NADPH regeneration system and terminated by addition of 800 µL ice cold 100% methanol (v/v) containing 1 µM chlorpropamide as internal standard. Then mixtures were kept in -20°C for 30 mins followed by centrifugation at 12,000 × g for 20 min at 4°C. The supernatants were dried in a under vacuum and reconstituted in 60 μL 50% methanol (v/v). After centrifugation, supernatants were transferred to autosampler vials for LC-MS/MS analysis. |
Combined analysis:
| Analysis ID | AN004051 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu 20AD |
| Column | BEH C18 column (2.1 × 100 mm × 1.7 µm particle size) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | ABI Sciex 5600 TripleTOF |
| Ion Mode | UNSPECIFIED |
| Units | 1 |
Chromatography:
| Chromatography ID: | CH002998 |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | BEH C18 column (2.1 × 100 mm × 1.7 µm particle size) |
| Column Temperature: | 55 |
| Flow Gradient: | The initial condition were 97% A and 3 % B, increasing to 45% B at 10 min, 75% B at 12 min where it was held at 75% B until 17.5 min before returning to the initial conditions. |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 100% wate; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003798 |
| Analysis ID: | AN004051 |
| Instrument Name: | ABI Sciex 5600 TripleTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The capillary voltage was set at 5.5 kV in positive ion mode with a declustering potential of 80 V. The mass spectrometer was operated with a 100 ms TOF scan from 50 to 500 m/z, and 16 MS/MS product ion scans (100 ms) per duty cycle using a collision energy of 50 V with a 20 V spread. |
| Ion Mode: | UNSPECIFIED |
