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MB Sample ID: SA248452
| Local Sample ID: | E.148 |
| Subject ID: | SU002572 |
| Subject Type: | Fungi |
| Subject Species: | Alternaria/Aspergillus/Botrytis/Anthostomella/Claussenomyces/Devriesia/Godronia/Leptodontidium/Mycosphaerella/Protoventuria/Rhizosphaera/Spondycladiopsis/Tumularia/Cladosporium/Colletotrichum/Coniochaeta/Coniothyrium/Creosphaeria/Cryptosporella/Cytospora/Diaporthe/Didymella/Discostroma/Epicoccum/Fusarium/Geastrumia/Gloeophyllum/Guignardia/Herpotrichia/Hypoxylon/Kretzschmaria/Lachnellula/Lachnum/Lophiostoma/Microdochium/Mollisia/Nemania/Nigrospora/Paramycosphaerella/Paraphaeosphaeria/Penicillium/Phaeoacremonium/Phialocephala/Phomopsis/Phyllosticta/Pilidium/Plagiostoma/Pleonectria/Proliferodiscus/Pseudoplectania/Pyrenochaeta/Pyrenopeziza/Ramularia/Rhytismataceae/Sarea/Seimatosporium/Setomelanomma/Sphaerulina/Stemphylium/Trichaptum/Varicosporium/Venturia/Wettsteinina/Xylaria |
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Subject:
| Subject ID: | SU002572 |
| Subject Type: | Fungi |
| Subject Species: | Alternaria/Aspergillus/Botrytis/Anthostomella/Claussenomyces/Devriesia/Godronia/Leptodontidium/Mycosphaerella/Protoventuria/Rhizosphaera/Spondycladiopsis/Tumularia/Cladosporium/Colletotrichum/Coniochaeta/Coniothyrium/Creosphaeria/Cryptosporella/Cytospora/Diaporthe/Didymella/Discostroma/Epicoccum/Fusarium/Geastrumia/Gloeophyllum/Guignardia/Herpotrichia/Hypoxylon/Kretzschmaria/Lachnellula/Lachnum/Lophiostoma/Microdochium/Mollisia/Nemania/Nigrospora/Paramycosphaerella/Paraphaeosphaeria/Penicillium/Phaeoacremonium/Phialocephala/Phomopsis/Phyllosticta/Pilidium/Plagiostoma/Pleonectria/Proliferodiscus/Pseudoplectania/Pyrenochaeta/Pyrenopeziza/Ramularia/Rhytismataceae/Sarea/Seimatosporium/Setomelanomma/Sphaerulina/Stemphylium/Trichaptum/Varicosporium/Venturia/Wettsteinina/Xylaria |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| E.148 | SA248452 | FL031283 | Sample | Type |
| E.148 | SA248452 | FL031283 | Nemania | Genus |
| E.148 | SA248452 | FL031283 | Nemania sp. | Full name |
| E.148 | SA248452 | FL031283 | Grape | Host |
Collection:
| Collection ID: | CO002565 |
| Collection Summary: | Endophyte samples were isolated from leaves and stems of Canadian fruit crops grown in Ontario, Nova Scotia, and New Brunswick between 2011 and 2015, according to protocol described in Endophytic Fungi in Vaccinium Macrocarpon (cranberry) and Vaccinium Angustifolium (blueberry) (Master's thesis by Ginn, 1998). Fruit crops include blueberries, raspberries, cranberries, and pears. |
| Sample Type: | Plant |
Treatment:
| Treatment ID: | TR002584 |
| Treatment Summary: | Fungal strains were grown on Potato Dextrose Agar (PDA) by streaking with homegenated liquid culture and were incubated in the dark at 25 C for at least 10 days, or until cultures achieved confluence. |
Sample Preparation:
| Sampleprep ID: | SP002578 |
| Sampleprep Summary: | Cultures were extracted by homogenizing the entire agar plate with 20 mL of methanol. Homogenates were filtered through Whatman #4 filter paper, and 100 uL aliquots were taken and diluted into 1 mL methanol. 10-1 dilutions of these prepared samples were analyzed by mass spectrometry (posmode ESI). |
Combined analysis:
| Analysis ID | AN004052 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity II |
| Column | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Integrated peak area |
Chromatography:
| Chromatography ID: | CH002999 |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
| Column Temperature: | 35 |
| Flow Gradient: | 0% B for 0.5 min, increase to 100% B over 3 min, held for 2.5 min, decrease to 0% B over 0.5 min, hold for 1 min |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% acetonitrile; 0.1% formic acid |
| Solvent B: | 100% water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003799 |
| Analysis ID: | AN004052 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data were acquired over a scan range of 100-1500 m/z at a resolution of 70,000, then the top 10 ions were selected and fragmented at a resolution of 17,500 Data were processed using xcms package in R free statistical software. |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 400 C |
| Capillary Voltage: | 3.9 kV |
| Collision Energy: | 28/50 |
