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MB Sample ID: SA251375
Local Sample ID: | WT1p_11_FA |
Subject ID: | SU002600 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002600 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
WT1p_11_FA | SA251375 | FL032018 | Wild-type | Genotype |
WT1p_11_FA | SA251375 | FL032018 | Pos | Treatment |
Collection:
Collection ID: | CO002593 |
Collection Summary: | Samples were collected and aliquots representing the same EV counts were dried under nitrogen, and resuspended in 2:2:1 acetonitrile:methanol:H2O (v/v) (for polar metabolites, eg. fatty acids, phospholipids) or in 100% isopropanol (for less-polar metabolites, eg. ceramides). The samples were mixed on an orbital shaker (360 rpm) for 1 min at room temperature before the LC/MS analysis. |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR002612 |
Treatment Summary: | Myotube Treatment with hMEC Generated FA-sEV: hMECs grown to confluence were serum-starved and treated with OA:BSA (100µM:50µM) or BSA (50µM controls). The sEVs were isolated from media collected over 48h and particle number and protein content determined. |
Sample Preparation:
Sampleprep ID: | SP002606 |
Sampleprep Summary: | Samples were collected and aliquots representing the same EV counts were dried under nitrogen, and resuspended in 2:2:1 acetonitrile:methanol:H2O (v/v) (for polar metabolites, eg. fatty acids, phospholipids) or in 100% isopropanol (for less-polar metabolites, eg. ceramides). The samples were mixed on an orbital shaker (360 rpm) for 1 min at room temperature before the LC/MS analysis. |
Combined analysis:
Analysis ID | AN004112 | AN004113 | AN004114 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | Reversed phase | HILIC | HILIC |
Chromatography system | Agilent 1290 Infinity II | Thermo Vanquish | Thermo Vanquish |
Column | Waters CORTECS UPLC C18 (150 x 2.1mm,1.6um) | Waters CORTECS HILIC (100 x 2.1mm,1.6um) | Waters CORTECS HILIC (100 x 2.1mm,1.6um) |
MS Type | ESI | ESI | ESI |
MS instrument type | QTOF | Orbitrap | Orbitrap |
MS instrument name | Agilent 6545 QTOF | Thermo Orbitrap ID-X Tribrid | Thermo Orbitrap ID-X Tribrid |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
Units | Peak Area | Peak Area | Peak Area |
Chromatography:
Chromatography ID: | CH003047 |
Methods Filename: | LC_Method.pdf |
Instrument Name: | Agilent 1290 Infinity II |
Column Name: | Waters CORTECS UPLC C18 (150 x 2.1mm,1.6um) |
Column Temperature: | 60 |
Flow Gradient: | 0-2 min, 30% B; 17 min, 75% B; 20 min, 85% B; 23-26 min, 100% B; 26 min, 30% B followed by a re-equilibration phase of 5 min. |
Flow Rate: | .25ml/min |
Solvent A: | A: 60% acetonitrile, 40% water, 0.1% formic acid, 10 mM ammonium formate, 2.5 mM medronic acid |
Solvent B: | 90% 2-propanol, 10% acetonitrile, 0.1% formic acid, 10 mM ammonium formate |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003048 |
Methods Filename: | LC_Method.pdf |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters CORTECS HILIC (100 x 2.1mm,1.6um) |
Column Temperature: | 45 |
Flow Gradient: | 0 to 1 min, 90% B; 12 min, 35% B; 12.5 to 14.5 min, 25% B; 15 min, 90% B followed by a re-equilibration phase of 4 |
Flow Rate: | .25ml/min |
Solvent A: | A: 95% water, 5% acetonitrile, 20 mM ammonium bicarbonate, 0.1% ammonium hydroxide solution (25% ammonia in water), 2.5 μM medronic acid |
Solvent B: | B: 95% acetonitrile, 5% water, 2.5 μM medronic acid |
Chromatography Type: | HILIC |
MS:
MS ID: | MS003859 |
Analysis ID: | AN004112 |
Instrument Name: | Agilent 6545 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | POSITIVE |
Analysis Protocol File: | MS_Method.pdf |
MS ID: | MS003860 |
Analysis ID: | AN004113 |
Instrument Name: | Thermo Orbitrap ID-X Tribrid |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | POSITIVE |
Analysis Protocol File: | MS_Method.pdf |
MS ID: | MS003861 |
Analysis ID: | AN004114 |
Instrument Name: | Thermo Orbitrap ID-X Tribrid |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | MS_Method.pdf |