Return to study ST002523 main page
MB Sample ID: SA254503
| Local Sample ID: | MK115 |
| Subject ID: | SU002623 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 68 (63, 73) |
| Weight Or Weight Range: | 77 (62, 87) |
| Height Or Height Range: | 170 (160, 176) |
| Gender: | Male and female |
| Human Ethnicity: | White |
| Human Medications: | anti-PD-1 immune checkpoint inhibitors (nivolumab or pembrolizumab) |
| Human Inclusion Criteria: | consecutive advanced cancer patients |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002623 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 68 (63, 73) |
| Weight Or Weight Range: | 77 (62, 87) |
| Height Or Height Range: | 170 (160, 176) |
| Gender: | Male and female |
| Human Ethnicity: | White |
| Human Medications: | anti-PD-1 immune checkpoint inhibitors (nivolumab or pembrolizumab) |
| Human Inclusion Criteria: | consecutive advanced cancer patients |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| MK115 | SA254503 | FL032603 | Low | Candida |
| MK115 | SA254503 | FL032603 | Female | Gender |
| MK115 | SA254503 | FL032603 | Nivolumab | Immunotherapy |
Collection:
| Collection ID: | CO002616 |
| Collection Summary: | Human urine samples were collected by from lung cancer patients and frozen at -80 °C. Samples were shipped to MS-OMICS for metabolomic analysis. |
| Sample Type: | Urine |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002635 |
| Treatment Summary: | To unfreeze samples at MS-OMICS, samples were assigned to their SpinX filters and centrifugated at 15,000 RPM for 5 minutes at 4 °C. Urine samples were then diluted 11 times in mobile phase eluent A and fortified with stable isotope labelled standards before analysis. |
| Treatment Compound: | mobile phase eluent A |
Sample Preparation:
| Sampleprep ID: | SP002629 |
| Sampleprep Summary: | Urine samples were then diluted 11 times in mobile phase eluent A and fortified with stable isotope labelled standards before analysis. |
Combined analysis:
| Analysis ID | AN004157 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | Thermo Orbitrap Exploris 240 |
| Ion Mode | UNSPECIFIED |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003076 |
| Chromatography Summary: | The samples were analysed with our semi-polar metabolites method, which is a slightly modified version of the protocol described by Catalin et al. (UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes, Water Application note 2011, 720004042en). The analysis was carried out in a randomised order using a UPLC system (Vanquish, Thermo Fisher Scientific) coupled with a high-resolution quadrupole-orbitrap mass spectrometer Orbitrap Exploris 240 MS, Thermo Fisher Scientific. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Gradient: | 300 μL/min: 0-2 min: 0% B, 2-12 min: 35% B, 12-13 min: 90% B, 13-14 min: 90% B, 14-15 min: 0% B |
| Flow Rate: | 300 μL/min |
| Solvent A: | 100% water; 10 mM ammonium formate; 0.1% formic acid (pH 3.1) |
| Solvent B: | 100% methanol; 10 mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003904 |
| Analysis ID: | AN004157 |
| Instrument Name: | Thermo Orbitrap Exploris 240 |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | The ionization was achieved with an electrospray ionization interface operated in positive and negative ionization mode under polarity switching. Data were processed using Compound Discoverer 3.3 (Thermo Fisher Scientific) and Skyline 21.2. |
| Ion Mode: | UNSPECIFIED |
