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MB Sample ID: SA260996
Local Sample ID: | 90430016811 |
Subject ID: | SU002717 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002717 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
90430016811 | SA260996 | FL033864 | Training | Group |
90430016811 | SA260996 | FL033864 | 1 week of training or control time | Timepoint |
90430016811 | SA260996 | FL033864 | Male | Sex |
Collection:
Collection ID: | CO002710 |
Collection Summary: | - |
Sample Type: | Liver |
Treatment:
Treatment ID: | TR002727 |
Treatment Summary: | - |
Sample Preparation:
Sampleprep ID: | SP002723 |
Sampleprep Summary: | Nucleotides are extracted from 200”l of tissue homogenates prepared at 50 mg /ml in methanol using hexane. The extracts are dried under nitrogen and reconstituted in water. |
Sampleprep Protocol Filename: | pass1b_experimental_design_metabolomics.pdf |
Combined analysis:
Analysis ID | AN004287 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity UPLC |
Column | Chromolith FastGradient RP-18e 50-2mm column (EMD Millipore, Billerica, MA, USA) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Waters Xevo TQ-XS |
Ion Mode | NEGATIVE |
Units | pmol/mg of tissue |
Chromatography:
Chromatography ID: | CH003193 |
Chromatography Summary: | Injection volume is 2 ”L. Mobile phase A is 95% water, 5% methanol and 5 mM dimethylhexylamine adjusted to pH 7.5 with acetic acid. Mobile phase B is 20% water, 80% methanol and 10 mM dimethylhexylamine. Flow rate is set to 0.3 mL/min and column temperature is 40 °C. A 22 min gradient method (t=0, %B=0; t=1.2, %B=0; t=22, %B=40) is run followed by a 3 min wash and 7 min equilibration. |
Methods Filename: | pass1b_nuc_methods.pdf |
Instrument Name: | Waters Acquity UPLC |
Column Name: | Chromolith FastGradient RP-18e 50-2mm column (EMD Millipore, Billerica, MA, USA) |
Column Temperature: | 40C |
Flow Gradient: | 22-minute gradient (t=0, %B=0; t=1.2, %B=0; t=22, %B=40) followed by a 3-minute wash and 7-minute equilibration |
Flow Rate: | 0.3 ml/min |
Solvent A: | 95% water/5% methanol; 5 mM dimethylhexylamine adjusted to pH 7.5 with acetic acid |
Solvent B: | 20% water/80% methanol; 10 mM dimethylhexylamine |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004034 |
Analysis ID: | AN004287 |
Instrument Name: | Waters Xevo TQ-XS |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | All nucleotides were detected in negative ion MRM mode based on a characteristic fragmentation reaction. |
Ion Mode: | NEGATIVE |