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MB Sample ID: SA261165
| Local Sample ID: | 90581016910 |
| Subject ID: | SU002720 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002720 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 90581016910 | SA261165 | FL033899 | Training | Group |
| 90581016910 | SA261165 | FL033899 | 2 weeks of training | Timepoint |
| 90581016910 | SA261165 | FL033899 | Female | Sex |
Collection:
| Collection ID: | CO002713 |
| Collection Summary: | - |
| Sample Type: | Brown adipose |
Treatment:
| Treatment ID: | TR002730 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP002726 |
| Sampleprep Summary: | homogenized in PBS, isotope IS added, deproteinized |
| Sampleprep Protocol Filename: | pass1b_experimental_design_metabolomics.pdf |
Combined analysis:
| Analysis ID | AN004290 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity UPLC |
| Column | Waters Acquity UPLC BEH C8 (150 x 2.1mm, 1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo TSQ Quantiva |
| Ion Mode | POSITIVE |
| Units | nano moles per vial |
Chromatography:
| Chromatography ID: | CH003196 |
| Chromatography Summary: | Analytes were chromatographically separated with 40 minutes LC gradient |
| Methods Filename: | pass1b_amines_methods.pdf |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | Waters Acquity UPLC BEH C8 (150 x 2.1mm, 1.7um) |
| Column Temperature: | 43C |
| Flow Gradient: | t=0 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=1 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=2 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=5.5 min: 98.1%/1.9% (%A/%B) @ 0.4 ml/min | t=6.5 min: 98%/2% (%A/%B) @ 0.4 ml/min | t=10 min: 97.5%/2.4% (%A/%B) @ 0.4 ml/min | t=12 min: 96%/4% (%A/%B) @ 0.4 ml/min | t=20 min: 88%/12% (%A/%B) @ 0.4 ml/min | t=27 min: 86.5%/13.5% (%A/%B) @ 0.4 ml/min | t=31 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=32 min: 2%/98% (%A/%B) @ 0.5 ml/min | t=35 min: 2%/98% (%A/%B) @ 0.5 ml/min | t=35.5 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min | t=39 min: 99.9%/0.1% (%A/%B) @ 0.4 ml/min |
| Flow Rate: | 0.4 ml/min; 0.5 ml/min |
| Solvent A: | 1% acetonitrile/99% water; 0.1% formic acid |
| Solvent B: | 1% water/99% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004037 |
| Analysis ID: | AN004290 |
| Instrument Name: | Thermo TSQ Quantiva |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Multiple reaction monitoring mode (MRM); peak integration, concentrations calculated against multi-point standard curves; |
| Ion Mode: | POSITIVE |
