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MB Sample ID: SA261595
Local Sample ID: | QC_29 |
Subject ID: | SU002728 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002728 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
QC_29 | SA261595 | FL033993 | QC-Pooled | Group |
QC_29 | SA261595 | FL033993 | 0 hour | Timepoint |
QC_29 | SA261595 | FL033993 | - | Sex |
Collection:
Collection ID: | CO002721 |
Collection Summary: | - |
Sample Type: | White adipose |
Treatment:
Treatment ID: | TR002737 |
Treatment Summary: | - |
Sample Preparation:
Sampleprep ID: | SP002734 |
Sampleprep Summary: | purification of oxylipids with SPE column |
Sampleprep Protocol Filename: | pass1b_experimental_design_metabolomics.pdf |
Combined analysis:
Analysis ID | AN004298 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | ExionLC AC |
Column | ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex 5500 Qtrap |
Ion Mode | NEGATIVE |
Units | ng/uL |
Chromatography:
Chromatography ID: | CH003204 |
Chromatography Summary: | Lipids were separated by liquid chromatography with 20 minutes gradient |
Methods Filename: | pass1b_oxylipneg_etamidpos_methods.pdf |
Instrument Name: | ExionLC AC |
Column Name: | ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um) |
Column Temperature: | 50C |
Flow Gradient: | t=0 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=0.5 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=1 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2.1 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=5 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=7 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12.1 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=18 min: 90%/10% (%A/%B) @ 0.5 ml/min |
Flow Rate: | 0.5 ml/min |
Solvent A: | 100% water; 10 mM ammonium acetate |
Solvent B: | 100% acetonitrile; 10 mM ammonium acetate |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004045 |
Analysis ID: | AN004298 |
Instrument Name: | ABI Sciex 5500 Qtrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Multiple reaction monitoring mode (MRM) |
Ion Mode: | NEGATIVE |