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MB Sample ID: SA262271
Local Sample ID: | 90441015206 |
Subject ID: | SU002737 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002737 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
90441015206 | SA262271 | FL034160 | Training | Group |
90441015206 | SA262271 | FL034160 | 2 weeks of training | Timepoint |
90441015206 | SA262271 | FL034160 | Male | Sex |
Collection:
Collection ID: | CO002730 |
Collection Summary: | - |
Sample Type: | Hippocampus |
Treatment:
Treatment ID: | TR002746 |
Treatment Summary: | - |
Sample Preparation:
Sampleprep ID: | SP002743 |
Sampleprep Summary: | See SOP |
Sampleprep Protocol Filename: | pass1b_experimental_design_metabolomics.pdf |
Combined analysis:
Analysis ID | AN004307 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1290 Infinity II |
Column | Agilent ZORBAX RRHD Extend 80Ĺ C18, 2.1 x 150 mm, 1.8 µm, 1200 bar |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6530 QTOF |
Ion Mode | NEGATIVE |
Units | peak area |
Chromatography:
Chromatography ID: | CH003213 |
Methods Filename: | pass1b_rpneg_rppos_ionpneg_methods.pdf |
Instrument Name: | Agilent 1290 Infinity II |
Column Name: | Agilent ZORBAX RRHD Extend 80Ĺ C18, 2.1 x 150 mm, 1.8 µm, 1200 bar |
Column Temperature: | 35C |
Flow Gradient: | t=0 min: 100%/0% (%A/%B) @ 0.25 ml/min | t=2 min: 100%/0% (%A/%B) @ 0.25 ml/min | t=12 min: 1%/99% (%A/%B) @ 0.25 ml/min | t=18 min: 1%/99% (%A/%B) @ 0.25 ml/min | t=28 min: 100%/0% (%A/%B) @ 0.40 ml/min | t=29.9 min: 100%/0% (%A/%B) @ 0.40 ml/min | t=30 min: 100%/0% (%A/%B) @ 0.25 ml/min |
Flow Rate: | 0.25 ml/min; 0.4 ml/min |
Solvent A: | 97% water/3% methanol; 15 mM tributylamine; 10 mM acetic |
Solvent B: | 100% methanol; 15 mM tributylamine; 10 mM acetic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004054 |
Analysis ID: | AN004307 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | See SOP protocol MiCAS_SOP004_Data post-processing and QC_v2_20190904.pdf |
Ion Mode: | NEGATIVE |