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MB Sample ID: SA264160
Local Sample ID: | 90266015810 |
Subject ID: | SU002763 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002763 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
90266015810 | SA264160 | FL034506 | Control | Group |
90266015810 | SA264160 | FL034506 | 8 weeks of training or control time | Timepoint |
90266015810 | SA264160 | FL034506 | Female | Sex |
Collection:
Collection ID: | CO002756 |
Collection Summary: | - |
Sample Type: | Heart |
Treatment:
Treatment ID: | TR002772 |
Treatment Summary: | - |
Sample Preparation:
Sampleprep ID: | SP002769 |
Sampleprep Summary: | 500 ul of tissue homogenate preprared at 50 mg/ml in 50% acetonitrile/0.3% formic acid are spiked with C17 Acyl CoA. The acyl CoAs are purified by solid phase extraction using 2-(2-pyridyl) ethyl functionalized Silica gel (Sigma-Aldrich 54127-U). |
Sampleprep Protocol Filename: | pass1b_experimental_design_metabolomics.pdf |
Combined analysis:
Analysis ID | AN004333 |
---|---|
Analysis type | MS |
Chromatography type | Flow induction analysis |
Chromatography system | Waters Acquity UPLC |
Column | No column |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Waters Xevo TQ-S |
Ion Mode | POSITIVE |
Units | pmol/mg of tissue |
Chromatography:
Chromatography ID: | CH003239 |
Chromatography Summary: | 80% methanol/20% water containing 30 mM ammonium hydroxide is used as the mobile phase, the flow is 0.030 ml/min. |
Methods Filename: | pass1b_acoa_methods.pdf |
Instrument Name: | Waters Acquity UPLC |
Column Name: | No column |
Chromatography Type: | Flow induction analysis |
MS:
MS ID: | MS004080 |
Analysis ID: | AN004333 |
Instrument Name: | Waters Xevo TQ-S |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Ion ratios of endogenous acyl CoAs to the C-17 CoA internal standard are computed from centroided spectra using a software package NeoLynx (Waters, Milford, MA). The ratios are converted to concentrations using calibrators prepared by spiking tissue homogenates with authentic CoAs (Sigma, St. Louis , MO) having saturated acyl chain lengths C0- C18. Corrections for the heavy isotope effects, mainly 13C, to the adjacent m+2 spectral peaks in a particular chain length cluster are made empirically by referring to the observed spectra for the analytical standards. The values are expressed in pmol/mg. Spectra are acquired in the multichannel acquisition mode monitoring the neutral loss of 507 amu (phosphoadenosine diphosphate) and scanning from m/z 750 to1060. |
Ion Mode: | POSITIVE |