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MB Sample ID: SA272988
Local Sample ID: | MMG-390_ABC-10628 |
Subject ID: | SU002816 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002816 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
MMG-390_ABC-10628 | SA272988 | FL035342 | Male | Sex |
MMG-390_ABC-10628 | SA272988 | FL035342 | CONTROL | Diagnosis |
Collection:
Collection ID: | CO002809 |
Collection Summary: | spun inside centrifuge and processed |
Sample Type: | Blood (plasma) |
Treatment:
Treatment ID: | TR002825 |
Treatment Summary: | NA |
Sample Preparation:
Sampleprep ID: | SP002822 |
Sampleprep Summary: | SOP blood-LCGCextract-02252019 |
Combined analysis:
Analysis ID | AN004393 | AN004394 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
Column | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6530 QTOF | Agilent 6550 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | normalized peak heights | normalized peak heights |
Chromatography:
Chromatography ID: | CH003296 |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
Column Temperature: | 45°C |
Flow Gradient: | 0 min, 100% B; 0.5 min, 100% B; 1.95 min, 70% B; 2.55 min, 30% B; 3.15 min, 100% B; 3.8 min, 100% B |
Flow Rate: | 0.8 mL/min |
Solvent A: | 100% water; 10 mM ammonium formate + 0.125% formic acid, pH 3 |
Solvent B: | 95% acetonitrile/5% water; 10 mM ammonium formate; 0.125% formic acid, pH 3 |
Chromatography Type: | HILIC |
MS:
MS ID: | MS004142 |
Analysis ID: | AN004393 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | The quadrupole/time-of-flight (QTOF) mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 65–1700 in positive (Agilent 6530, equipped with a JetStreamSource) and negative (Agilent 6550, equipped with a dual JetStream Source) modes producing both unique and complementary spectra. Instrument parameters are as follows (positive mode) Gas Temp 325°C, Gas Flow 8 l/min, Nebulizer 35 psig, Sheath Gas 350°C, Sheath Gas Flow 11, Capillary Voltage 3500 V, Nozzle Voltage 1000V, Fragmentor 120V, Skimmer 65V. Data (both profile and centroid) are collected at a rate of 2 scans per second. In negative ion mode, Gas Temp 200°C, Gas Flow 14 l/min, Fragmentor 175V, with the other parameters identical to positive ion mode. For the 6530 QTOF, a reference solution generating ions of 121.050 and 922.007 m/z in positive mode and 119.036 and 966.0007 m/z in negative mode, and these are used for continuous mass correction. For the 6550, the reference solution is introduced via a dual spray ESI, with the same ions and continuous mass correction |
Ion Mode: | POSITIVE |
MS ID: | MS004143 |
Analysis ID: | AN004394 |
Instrument Name: | Agilent 6550 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | The quadrupole/time-of-flight (QTOF) mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 65–1700 in positive (Agilent 6530, equipped with a JetStreamSource) and negative (Agilent 6550, equipped with a dual JetStream Source) modes producing both unique and complementary spectra. Instrument parameters are as follows (positive mode) Gas Temp 325°C, Gas Flow 8 l/min, Nebulizer 35 psig, Sheath Gas 350°C, Sheath Gas Flow 11, Capillary Voltage 3500 V, Nozzle Voltage 1000V, Fragmentor 120V, Skimmer 65V. Data (both profile and centroid) are collected at a rate of 2 scans per second. In negative ion mode, Gas Temp 200°C, Gas Flow 14 l/min, Fragmentor 175V, with the other parameters identical to positive ion mode. For the 6530 QTOF, a reference solution generating ions of 121.050 and 922.007 m/z in positive mode and 119.036 and 966.0007 m/z in negative mode, and these are used for continuous mass correction. For the 6550, the reference solution is introduced via a dual spray ESI, with the same ions and continuous mass correction |
Ion Mode: | NEGATIVE |