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MB Sample ID: SA273930
| Local Sample ID: | AO_03 |
| Subject ID: | SU002830 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002830 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| AO_03 | SA273930 | FL035522 | Control | Expression vector |
Collection:
| Collection ID: | CO002823 |
| Collection Summary: | ATCC HeLa - CCL-2 |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR002839 |
| Treatment Summary: | Transfection with SPMD3 gene |
Sample Preparation:
| Sampleprep ID: | SP002836 |
| Sampleprep Summary: | Ceramides were extracted using 2:3 70% Isopropanol: EthylAcetate. See protocol.docx for details. |
| Processing Storage Conditions: | -20℃ |
Combined analysis:
| Analysis ID | AN004415 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Spectra C8SR (150 × 3.0 mm, 3um) (#S-3C8SR-FJ) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantiva |
| Ion Mode | POSITIVE |
| Units | pmols |
Chromatography:
| Chromatography ID: | CH003314 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Spectra C8SR (150 × 3.0 mm, 3um) (#S-3C8SR-FJ) |
| Column Temperature: | 37 |
| Flow Gradient: | 0.5ml/min. 70%B. 5 min 90%B, 17 min 99%B, 26min 99%B, 26.5min 70% B. Stop at 35min. |
| Flow Rate: | 0.5ml/min |
| Solvent A: | 100% water; 0.2% formic acid ; 1mM ammonium formate |
| Solvent B: | 100% methanol; 0.2% formic acid ; 1mM ammonium formate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004162 |
| Analysis ID: | AN004415 |
| Instrument Name: | Thermo Quantiva |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Acquisition and Processing were done using TraceFinder Software. |
| Ion Mode: | POSITIVE |
