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MB Sample ID: SA273933
Local Sample ID: | AO_13 |
Subject ID: | SU002830 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002830 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
AO_13 | SA273933 | FL035523 | nSMase2-V5 | Expression vector |
Collection:
Collection ID: | CO002823 |
Collection Summary: | ATCC HeLa - CCL-2 |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR002839 |
Treatment Summary: | Transfection with SPMD3 gene |
Sample Preparation:
Sampleprep ID: | SP002836 |
Sampleprep Summary: | Ceramides were extracted using 2:3 70% Isopropanol: EthylAcetate. See protocol.docx for details. |
Processing Storage Conditions: | -20℃ |
Combined analysis:
Analysis ID | AN004415 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Spectra C8SR (150 × 3.0 mm, 3um) (#S-3C8SR-FJ) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo Quantiva |
Ion Mode | POSITIVE |
Units | pmols |
Chromatography:
Chromatography ID: | CH003314 |
Instrument Name: | Thermo Vanquish |
Column Name: | Spectra C8SR (150 × 3.0 mm, 3um) (#S-3C8SR-FJ) |
Column Temperature: | 37 |
Flow Gradient: | 0.5ml/min. 70%B. 5 min 90%B, 17 min 99%B, 26min 99%B, 26.5min 70% B. Stop at 35min. |
Flow Rate: | 0.5ml/min |
Solvent A: | 100% water; 0.2% formic acid ; 1mM ammonium formate |
Solvent B: | 100% methanol; 0.2% formic acid ; 1mM ammonium formate |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004162 |
Analysis ID: | AN004415 |
Instrument Name: | Thermo Quantiva |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Acquisition and Processing were done using TraceFinder Software. |
Ion Mode: | POSITIVE |