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MB Sample ID: SA288793
| Local Sample ID: | KO3_001 |
| Subject ID: | SU002846 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 26 weeks |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002846 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 26 weeks |
| Gender: | Female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| KO3_001 | SA288793 | FL035613 | Lmna Knock-Out | genotype |
Collection:
| Collection ID: | CO002839 |
| Collection Summary: | Murine brains were harvested at 26 weeks and were snap-frozen for 5 min on an aluminum boat floating on liquid nitrogen. |
| Sample Type: | Brain |
Treatment:
| Treatment ID: | TR002855 |
| Treatment Summary: | The experimental group was composed of three 26-week-old female mice with conditional ablation of Lmna in the oligodendrocytes (CnpCre/+;Lmnafl/fl). The control group consisted of three wildtype 26-week-old female mice |
Sample Preparation:
| Sampleprep ID: | SP002852 |
| Sampleprep Summary: | A coronal slice of brain (approximate 15 mg) at (-0.88 mm to -1.335mm from Bregma) plane was sectioned and homogenized in cold Methanol/Water (80/20, v/v) to a final concentration of 30mg/ml. Following 20 min of gentle sonication in Bioruptor (30 s on, 30 s off, 20 cycles) at 4 °C, samples were centrifuged for 10 min at 10,000xg at 4 °C and were processed for LC-MS/MS |
Combined analysis:
| Analysis ID | AN004442 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Bruker maXis-II |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH003337 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
| Column Temperature: | 30 |
| Flow Gradient: | 0.15mL/min; 0-5.0 min; 2.0% B, 5.0-28.0 min; 2.0-60.0% B, 28.0-38.0 min; 60.0% B, 38.0-39.0 min; 60.0-2.0% B, 39.0-48.0 min, 2.0% B. |
| Flow Rate: | 0.15mL/min |
| Solvent A: | 97% acetonitrile/3% water/7mM ammonium acetate |
| Solvent B: | 97% water/3% acetonitrile/7mM ammonium acetate |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004189 |
| Analysis ID: | AN004442 |
| Instrument Name: | Bruker maXis-II |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS spectra were obtained using a Bruker maXis-II-ETD UHR-ESI-QTOF, witb Ultra High Resolution QTOF (UHR) technology in addition to Electron-Transfer Dissociation (ETD). Compound identification and descriptive statistical analysis of the LC-MS/MS data were performed through Metaboscape and XCMSPlus software. Bruker MetaboBase Personal 3.0, MoNA, MSDIAL, METLIN, and HMDB metabolomic libraries were used in compound identification. Ultimately, both accurate mass-measurements (with less than 5 pmm accuracy) and fragmentation spectra (or simply MS/MS spectra) were used for confident identification of metabolites and lipids. |
| Ion Mode: | POSITIVE |
