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MB Sample ID: SA294323
| Local Sample ID: | 44 |
| Subject ID: | SU002877 |
| Subject Type: | Bacteria |
| Subject Species: | Bacillus pumilus |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002877 |
| Subject Type: | Bacteria |
| Subject Species: | Bacillus pumilus |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 44 | SA294323 | FL035924 | B2 | Group |
Collection:
| Collection ID: | CO002870 |
| Collection Summary: | The broth cultures were centrifuged at 7000 rpm for 10 min under 4 °C and the supernatants were collected. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR002886 |
| Treatment Summary: | The full-length 16S rRNA gene sequence was obtained for the studied Bacillus strains. The phylogenetic tree of the 16S rRNA gene showed that the strains formed two groups, which were defined as Group B1 and B2. The antagonistic activities of the Bacillus broth against the vp-HL strain were evaluated by the agar well diffusion method. The DI (diameters of the inhibition zones) values of the 20 Bacillus strains against the vp-HL strain were measured. All strains belonging to Group B2 showed DI values greater than those of strains from Group B1. |
Sample Preparation:
| Sampleprep ID: | SP002883 |
| Sampleprep Summary: | The broth cultures were centrifuged at 7000 rpm for 10 min under 4 °C and the supernatants were collected. Ethyl acetate (EtOAc) with 2 × volume was added and sonicated for 10 min. The samples were then centrifuged at 7000 rpm at 4 °C for 10 min and the organic phase was collected and dried using a rotary evaporator. The extracts were resuspended in 1:1 acetonitrile: H2O with 1% formic acid. These samples were diluted 20 times using methanol. All samples were filtrated through 0.22 µm syringe filters. |
Combined analysis:
| Analysis ID | AN004507 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Vion IMS QTof |
| Ion Mode | POSITIVE |
| Units | intensity |
Chromatography:
| Chromatography ID: | CH003387 |
| Chromatography Summary: | Low pH polar |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
| Column Temperature: | 30 |
| Flow Gradient: | 5%B was kept for 0.5 min, 0.5-2.5 min, 5% to 30% B; 2.5-3.5 min, 30% to 40% B; 3.5-9 min, 40% to 99% B; 9-10 min, 99% to 5% B; and 5%B was kept for 3 min. |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | Water with 0.1% (vol/vol) formic acid |
| Solvent B: | Acetonitrile with 0.1% (vol/vol) formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004254 |
| Analysis ID: | AN004507 |
| Instrument Name: | Waters Vion IMS QTof |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | UNIFI software (v. 1.8, Waters) |
| Ion Mode: | POSITIVE |
