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MB Sample ID: SA300167
Local Sample ID: | Salvia-semiatrata |
Subject ID: | SU002902 |
Subject Type: | Plant |
Subject Species: | Salvia |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002902 |
Subject Type: | Plant |
Subject Species: | Salvia |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Salvia-semiatrata | SA300167 | FL036444 | Salvia semiatrata | Taxon |
Collection:
Collection ID: | CO002895 |
Collection Summary: | Samples were collected from live mature plants and separated into root and leaf samples, with 3 biological replicates. Then samples were frozen in liquid nitrogen. The 2 parts of each plant were crushed into homogeneous powders respectively. In total, 71 species from three major distribution centers for this genus representing 8 out of the 11 recognized subclades of Salvia were sampled. To obtain sufficient materials for both chemical analyses, samples were collected at the vegetative or flowering stages. |
Sample Type: | Plant |
Treatment:
Treatment ID: | TR002911 |
Treatment Summary: | No treatment, just species difference. In total, 71 species from three major distribution centers for this genus representing 8 out of the 11 recognized subclades of Salvia were sampled. |
Sample Preparation:
Sampleprep ID: | SP002908 |
Sampleprep Summary: | All samples were ground into a fine powder, and 10 mg prepared powder was extracted with 1 mL of 70% methanol (v/v) containing warfarin (5 μg/mL) as the internal reference for 1 h in an ultrasonic bath (53 kHz, 350 W) at 4 °C. After centrifuging at 12,000 g at 4 °C for 30 min, the supernatant was used for UHPLC-QTOF-MS analysis. |
Combined analysis:
Analysis ID | AN004547 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Waters Xevo-G2-XS |
Ion Mode | UNSPECIFIED |
Units | Normalized peak height |
Chromatography:
Chromatography ID: | CH003416 |
Instrument Name: | Waters Acquity |
Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
Column Temperature: | 40 °C |
Flow Gradient: | The elution gradients were 98–80% A over 0–7 min, 80–78% A over 7–11 min, 78–40% A over 11–20 min, 40–35% A over 20–25 min, 25–28 min at 35% A, 35–5% A over 28–30 min, 30–33 min at 5% A, and final re-equilibration at 98% A for 5 min. |
Flow Rate: | 0.40 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004294 |
Analysis ID: | AN004547 |
Instrument Name: | Waters Xevo-G2-XS |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | MS-DIAL v4.60 software. The data in the results table represent the average values for roots and leaves. Positive and negative ion-mode data were combined in the results table |
Ion Mode: | UNSPECIFIED |