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MB Sample ID: SA300818
| Local Sample ID: | B55_018 |
| Subject ID: | SU002910 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | n/a |
| Age Or Age Range: | 3 weeks |
| Weight Or Weight Range: | not specified |
| Height Or Height Range: | not specified |
| Gender: | Male and female |
| Animal Housing: | regular housing |
| Animal Feed: | regular diet |
| Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002910 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | n/a |
| Age Or Age Range: | 3 weeks |
| Weight Or Weight Range: | not specified |
| Height Or Height Range: | not specified |
| Gender: | Male and female |
| Animal Housing: | regular housing |
| Animal Feed: | regular diet |
| Animal Water: | ad libitum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| B55_018 | SA300818 | FL036538 | F off-RD | Treatment Group |
Collection:
| Collection ID: | CO002903 |
| Collection Summary: | Bone marrow cells were isolated as previously reported (35). Briefly, femurs were flushed by 25G5/8 needle with medium containing RPMI1640+2% FBS+10 units/ml Heparin + penicillin and streptomycin. Bits of bones were removed using a sterile 4um nylon cell strainer (Falcon 352340). Cells were dissolved in 50 ml medium and centrifuged at 2000 rpm (900x g), for 10 minutes at 4oC. Cell pellets were then washed twice with 50 ml of serum-free RPMI (RPMI1640+20mM Hepes + penicillin and streptomycin, adjusted to pH 7.4 before filtering), centrifuged at 2000 rpm, at 4oC for 5 minutes, and resuspended in 25 ml of serum-free RPMI. |
| Sample Type: | Bone marrow |
Treatment:
| Treatment ID: | TR002919 |
| Treatment Summary: | To induce maternal obesity, high-fat diet (Teklad Cat#TD.06415) or its control, a regular diet (RD, PicoLab® Laboratory Rodent Diet Cat # 5L0D) was given to virgin female FVB/NJ mice from the six-weeks of age and throughout the entire study. After eight weeks of dietary intervention, RD- and HFD-fed female mice were bred to an age-matched RD-fed male. At weaning, male and female offspring from each mother were randomly selected for the study. The offspring were fed a regular diet only starting from weaning and throughout their life. |
Sample Preparation:
| Sampleprep ID: | SP002916 |
| Sampleprep Summary: | Full sample was extracted using Matyash extraction procedure which includes MTBE, MeOH, and H2O. The organic (upper) phase was dried down and submitted for resuspension and injection onto the LC while the aqueous (bottom) phase was dried down and submitted to derivatization for GC. |
Combined analysis:
| Analysis ID | AN004559 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco GC-TOF |
| Ion Mode | POSITIVE |
| Units | quant m/z |
Chromatography:
| Chromatography ID: | CH003426 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Temperature: | 275 |
| Flow Gradient: | - |
| Flow Rate: | 1 mL/min |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | GC |
MS:
| MS ID: | MS004306 |
| Analysis ID: | AN004559 |
| Instrument Name: | Leco GC-TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | Scan range of m/z 120-1200 Da with an acquisition speed of 2 spectra/s. |
| Ion Mode: | POSITIVE |
