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MB Sample ID: SA307617
Local Sample ID: | Liver_Tglif_F_3F |
Subject ID: | SU002953 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002953 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Liver_Tglif_F_3F | SA307617 | FL037072 | TgLC | Genotype |
Liver_Tglif_F_3F | SA307617 | FL037072 | Fed | Condition |
Collection:
Collection ID: | CO002946 |
Collection Summary: | TgLC and TgL mice were injected with TAM. Balb/c mice were injected with C26 or C26-LIF KO cells. Liver was collected and then stored at -80 °C. |
Sample Type: | Liver |
Treatment:
Treatment ID: | TR002962 |
Treatment Summary: | TgL and TgLC mice were injected with Tamoxifen. Balb/c mice were injected with C26 or C26-LIF KO cells. |
Sample Preparation:
Sampleprep ID: | SP002959 |
Sampleprep Summary: | For lipidomic metabolites extraction, 10 mg homogenized tissues were mixed with 120 μL methanol and 400 μL MTBE. After shaking for 6 minutes at 4 °C, 100 μL H2O was added and followed by centrifugation for 2 minutes. Supernatants were dried down for further lipidomics analysis. |
Combined analysis:
Analysis ID | AN004652 | AN004653 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
Ion Mode | POSITIVE | POSITIVE |
Units | Ion counts | Ion counts |
Chromatography:
Chromatography ID: | CH003502 |
Chromatography Summary: | Lipidomics method |
Instrument Name: | Thermo Vanquish |
Column Name: | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
Column Temperature: | 55C |
Flow Gradient: | 0 min, 25% B; 2 min, 25% B; 5.5 min, 65% B; 12.5 min, 100% B; 19.5 min, 100% B; 20.0 min, 25% B; 30 min, 25% B |
Flow Rate: | 0.2mL/min |
Solvent A: | 90% water/10% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate |
Solvent B: | 75% isopropanol/25% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004399 |
Analysis ID: | AN004652 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Positive Ionization mode |
Ion Mode: | POSITIVE |
Capillary Temperature: | 300 |
Ion Source Temperature: | 360 |
Spray Voltage: | 2700 |
Automatic Gain Control: | 3000000 |
Acquisition Parameters File: | LCMS_Pos_RT_30min_55C_LongElution |
MS ID: | MS004400 |
Analysis ID: | AN004653 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Positive Ionization mode |
Ion Mode: | POSITIVE |
Capillary Temperature: | 300 |
Ion Source Temperature: | 360 |
Spray Voltage: | 2700 |
Automatic Gain Control: | 3000000 |
Acquisition Parameters File: | LCMS_Pos_RT_30min_55C_LongElution |