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MB Sample ID: SA314067
Local Sample ID: | A57 |
Subject ID: | SU002986 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002986 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
A57 | SA314067 | FL037437 | AAA | treatment |
Collection:
Collection ID: | CO002979 |
Collection Summary: | Plasma samples from 100 AAA patients and 100 non-AAA controls were collected from Beijing Anzhen Hospital of Capital Medical University and Tongji Medical College of Huazhong University of Science and Technology. |
Sample Type: | plasma |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002995 |
Treatment Summary: | Plasma samples from 100 AAA patients and 100 non-AAA controls were collected from Beijing Anzhen Hospital of Capital Medical University and Tongji Medical College of Huazhong University of Science and Technology. |
Sample Preparation:
Sampleprep ID: | SP002992 |
Sampleprep Summary: | Briefly, 50 μL plasma spiked with 10 µL internal standard mixture and 10 µL BHT was blended into 380 µL methanol (75%). MTBE (1 mL) was added, and the mixture was vortexed for 30 min. Phase separation was induced by adding 250 µL H₂O. After incubation for 10 min at room temperature, the samples were centrifuged at 14 000 g for 10 min. The upper phase was collected and dried using a nitrogen evaporator. Ultra-performance liquid chromatography BEH C18 columns (1.7 μm, 100 × 2.1 mm i.d.) were used for chromatographic separations. |
Combined analysis:
Analysis ID | AN004709 | AN004710 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Waters Acquity | Waters Acquity |
Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTRAP | QTRAP |
MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | ng/mL | ng/mL |
Chromatography:
Chromatography ID: | CH003546 |
Instrument Name: | Waters Acquity |
Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
Column Temperature: | 50 |
Flow Gradient: | 0.25mL/min:0-3min:25%A:75%B,3-17min:99%A:1%B, 17-20min:25%A:75%B |
Flow Rate: | 0.25mL/min |
Solvent A: | 90% isopropanol/10% acetonitrile; 5mM ammonium acetate |
Solvent B: | 60% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004455 |
Analysis ID: | AN004709 |
Instrument Name: | ABI Sciex 5500 QTrap |
Instrument Type: | QTRAP |
MS Type: | ESI |
MS Comments: | Raw data were processed using MultiQuant 3.0.3. Metabolite levels were calculated as the ratio of their concentrations relative to a standard sample. Quantifing the targeted metabolites by area under the curve used expected retention time. |
Ion Mode: | POSITIVE |
MS ID: | MS004456 |
Analysis ID: | AN004710 |
Instrument Name: | ABI Sciex 5500 QTrap |
Instrument Type: | QTRAP |
MS Type: | ESI |
MS Comments: | Raw data were processed using MultiQuant 3.0.3. Metabolite levels were calculated as the ratio of their concentrations relative to a standard sample. Quantifing the targeted metabolites by area under the curve used expected retention time. |
Ion Mode: | NEGATIVE |