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MB Sample ID: SA314466
Local Sample ID: | LAT1-4F2hc_WT_delipidated_1 |
Subject ID: | SU002988 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | HEK293F |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002988 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | HEK293F |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
LAT1-4F2hc_WT_delipidated_1 | SA314466 | FL037442 | Delipidated | Treatment |
Collection:
Collection ID: | CO002981 |
Collection Summary: | The LAT1-4F2hc complex was buffer-exchanged into 1M ammonium acetate, pH 7.0 with 2 mM OGNG and incubated with 1 µg trypsin overnight at 37 °C. The digested peptide/lipid mixture was dried using a SpeedVac vacuum concentrator (Thermo Fisher Scientific) |
Sample Type: | HEK cells |
Treatment:
Treatment ID: | TR002997 |
Treatment Summary: | The LAT1-4F2hc complex was buffer-exchanged into 1M ammonium acetate (pH 7.0) with 20 mM OGNG using a 100 kD MWCO centrifugal filter (Amicon Ultra-0.5 ml, Millipore) and incubated for 4 h at room temperature with gentle mixing. Excess lipomicelles and detergents were removed by buffer-exchanging into 1 M ammonium acetate with 2 mM OGNG using the same filter. |
Sample Preparation:
Sampleprep ID: | SP002994 |
Sampleprep Summary: | The lipid mixture was reconstituted with 70% mobile phase A (acetonitrile/H2O: 60/40, 10 mM ammonium formate and 0.1% formic acid) and 30% mobile phase B (isopropanol/acetonitrile: 90/10, 10 mM ammonium formate and 0.1% formic acid) for the following LC-MS/MS analysis. |
Combined analysis:
Analysis ID | AN004712 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Thermo Acclaim PepMap 100 C18 (150 x 0.007 mm, 3um, 100A) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo LTQ XL |
Ion Mode | NEGATIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH003548 |
Chromatography Summary: | Column information: https://www.thermofisher.com/order/catalog/product/164568 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Thermo Acclaim PepMap 100 C18 (150 x 0.007 mm, 3um, 100A) |
Column Temperature: | 40 |
Flow Gradient: | 30%-99% |
Flow Rate: | 300 nl/min |
Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid |
Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004458 |
Analysis ID: | AN004712 |
Instrument Name: | Thermo LTQ XL |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | NEGATIVE |