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MB Sample ID: SA317705
Local Sample ID: | d_AD_FA_79 |
Subject ID: | SU003037 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Not applicable |
Human Race: | Pacific asian |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003037 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Not applicable |
Human Race: | Pacific asian |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
d_AD_FA_79 | SA317705 | FL037818 | AD_FA | Factor |
Collection:
Collection ID: | CO003030 |
Collection Summary: | The study population consisted of 158 six-month-old infants (46 healthy infants, 30 only AD group, and 82 with combined AD and FA) involved in the Cohort for Childhood Origin of Asthma and Allergic Diseases (COCOA) (reference: BMC Pulm Med 2014, 14:109.) |
Sample Type: | Feces |
Treatment:
Treatment ID: | TR003046 |
Treatment Summary: | no treatment |
Sample Preparation:
Sampleprep ID: | SP003043 |
Sampleprep Summary: | ~40 mg of feces was used and lipids were extracted by Bligh and Dyer method after adding internal standard solutions (50 μl of 100 nM C18 ceramide-d7 and C18:1 Sphingomyelin-d9 and 200 μl of 1 μM 1,3-19:0-d5). Organic solutions containing the lipids were dried using a vacuum centrifuge and stored at –20°C until LC-MS/MS analysis. The dried matter was reconstituted with methanol and injected into the LC-MS/MS system. |
Combined analysis:
Analysis ID | AN004794 | AN004795 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
Column | Agilent Pursuit 5 C18 (150 x 2.0 mm, 5um) | Agilent Pursuit 5 C18 (150 x 2.0 mm, 5um) |
MS Type | ESI | ESI |
MS instrument type | QTRAP | QTRAP |
MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
Ion Mode | POSITIVE | POSITIVE |
Units | pmol/mg | pmol/mg |
Chromatography:
Chromatography ID: | CH003625 |
Chromatography Summary: | Ceramides, sphingomyelines, sphinganine, sphingosine |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Agilent Pursuit 5 C18 (150 x 2.0 mm, 5um) |
Column Temperature: | 35 |
Flow Gradient: | 50 % of B at 0 min, 50 % of B for 5 min, 50-70 % of B for 3 min, 70 % of B for 7 min, 70-90 % of B for 7 min, 90 % of B for 3 min, 90-50 % of B for 0.1 min, 50 % of B for 4.9 min |
Flow Rate: | 200 uL/min |
Solvent A: | 5 mM ammonium formate/MeOH/tetrahydrofuran (500/200/300, v/v/v) |
Solvent B: | 5 mM ammonium formate/MeOH/ tetrahydrofuran (100/200/700, v/v/v) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003626 |
Chromatography Summary: | diacylglycerols |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Agilent Pursuit 5 C18 (150 x 2.0 mm, 5um) |
Column Temperature: | 35 |
Flow Gradient: | 90 % of B for 0 min, 90 % of B for 6 min, 90 to 95 % of B for 0.6 min, 95 % of B for 3.4 min, 95 to 90 % of B for 0.1 min, and 90 % of B for 1.9 min |
Flow Rate: | 200 uL/min |
Solvent A: | 5 mM ammonium formate/MeOH/tetrahydrofuran (500/200/300, v/v/v) |
Solvent B: | 5 mM ammonium formate/MeOH/ tetrahydrofuran (100/200/700, v/v/v) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004540 |
Analysis ID: | AN004794 |
Instrument Name: | ABI Sciex 5500 QTrap |
Instrument Type: | QTRAP |
MS Type: | ESI |
MS Comments: | Multiple reaction monitoring (MRM) was performed in the positive ion mode and the extracted ion chromatogram corresponding to the specific transition for each lipid was used for quantification. The calibration range for each lipid was 0.1-1000 nM (r2 ≥ 0.99). Data analysis was performed by using Analyst 1.5.2 software. |
Ion Mode: | POSITIVE |
Acquisition Parameters File: | MS_metadata.docx |
MS ID: | MS004541 |
Analysis ID: | AN004795 |
Instrument Name: | ABI Sciex 5500 QTrap |
Instrument Type: | QTRAP |
MS Type: | ESI |
MS Comments: | Multiple reaction monitoring (MRM) was performed in the positive ion mode and the extracted ion chromatogram corresponding to the specific transition for each lipid was used for quantification. The calibration range for each lipid was 0.1-1000 nM (r2 ≥ 0.99). Data analysis was performed by using Analyst 1.5.2 software. |
Ion Mode: | POSITIVE |
Acquisition Parameters File: | MS_metadata.docx |