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MB Sample ID: SA321843
Local Sample ID: | 20_T0_3 |
Subject ID: | SU003067 |
Subject Type: | Plant |
Subject Species: | Thalassiosira pseudonana |
Taxonomy ID: | 296543 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003067 |
Subject Type: | Plant |
Subject Species: | Thalassiosira pseudonana |
Taxonomy ID: | 296543 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
20_T0_3 | SA321843 | FL038322 | 20 | Temperature |
20_T0_3 | SA321843 | FL038322 | 0 | Time point |
Collection:
Collection ID: | CO003060 |
Collection Summary: | 1 mL of subsamples were collected in 1.5-mL tubes at the incubation periods of 0 and 10 hours for NMR analyses. |
Collection Protocol Filename: | 2_Collection protocol_UGA_temp_Oct2023_drawdown.docx |
Sample Type: | Algae |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR003076 |
Treatment Summary: | Axenic cultures (1 L in L1 media, 35 ppt) of the diatom Thalassiosira pseudonana CCMP1335 were grown at different temperatures of 14, 20, and 28°C under 120 µmol photons m-2 s-1 (ULM-500 Light Meter, Walz) and a 16:8 h light:dark cycle (four replicates for each treatment). They were harvested in late exponential growth phase by filtration onto 2.0 µm Isopore filters (1 L) and stored at -20°C for later experimentation. Diatom cells were rinsed from filters into 10 mL sterile L1 media, sonicated to lyse cells for 20 min, and passed through a pre-combusted GF/F filter. R. pomeroyi was inoculated into 2.7 mL of the endometabolome samples (3 x 107 cells mL-1) and grown for 10 h. |
Treatment Protocol Filename: | 3_Treatment protocol_UGA_temp_Oct2023_drawdown.docx |
Sample Preparation:
Sampleprep ID: | SP003073 |
Sampleprep Summary: | 540-µL of subsamples were mixed with 60-µL of a phosphate buffer (30 mmol L-1 phosphate in deuterated water; pH 7.4; 1 mmol L-1 internal standard 2,2-dimethyl-2-silapentane-5-sulfonate) and transferred to NMR tubes. One media-blank sample was also included. |
Sampleprep Protocol Filename: | 4_Sample preparation protocol_UGA_temp_Oct2023_drawdown.docx |
Analysis:
MB Sample ID: | SA321843 |
Analysis ID: | AN004851 |
Analysis Type: | NMR |
Analysis Protocol File: | 5_Analysis protocol_UGA_temp_Oct2023_drawdown.docx |
Software Version: | TopSpin version 3.5 |
Num Factors: | 6 |
Num Metabolites: | 16 |
Units: | Intensity |
NMR:
NMR ID: | NM000268 |
Analysis ID: | AN004851 |
Instrument Name: | AVANCE III HD instrument (Bruker) |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 2D-1H-13C |
Spectrometer Frequency: | 600 MHz |