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MB Sample ID: SA321854
| Local Sample ID: | 28_T10_3 |
| Subject ID: | SU003067 |
| Subject Type: | Plant |
| Subject Species: | Thalassiosira pseudonana |
| Taxonomy ID: | 296543 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003067 |
| Subject Type: | Plant |
| Subject Species: | Thalassiosira pseudonana |
| Taxonomy ID: | 296543 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 28_T10_3 | SA321854 | FL038325 | 28 | Temperature |
| 28_T10_3 | SA321854 | FL038325 | 10 | Time point |
Collection:
| Collection ID: | CO003060 |
| Collection Summary: | 1 mL of subsamples were collected in 1.5-mL tubes at the incubation periods of 0 and 10 hours for NMR analyses. |
| Collection Protocol Filename: | 2_Collection protocol_UGA_temp_Oct2023_drawdown.docx |
| Sample Type: | Algae |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003076 |
| Treatment Summary: | Axenic cultures (1 L in L1 media, 35 ppt) of the diatom Thalassiosira pseudonana CCMP1335 were grown at different temperatures of 14, 20, and 28°C under 120 µmol photons m-2 s-1 (ULM-500 Light Meter, Walz) and a 16:8 h light:dark cycle (four replicates for each treatment). They were harvested in late exponential growth phase by filtration onto 2.0 µm Isopore filters (1 L) and stored at -20°C for later experimentation. Diatom cells were rinsed from filters into 10 mL sterile L1 media, sonicated to lyse cells for 20 min, and passed through a pre-combusted GF/F filter. R. pomeroyi was inoculated into 2.7 mL of the endometabolome samples (3 x 107 cells mL-1) and grown for 10 h. |
| Treatment Protocol Filename: | 3_Treatment protocol_UGA_temp_Oct2023_drawdown.docx |
Sample Preparation:
| Sampleprep ID: | SP003073 |
| Sampleprep Summary: | 540-µL of subsamples were mixed with 60-µL of a phosphate buffer (30 mmol L-1 phosphate in deuterated water; pH 7.4; 1 mmol L-1 internal standard 2,2-dimethyl-2-silapentane-5-sulfonate) and transferred to NMR tubes. One media-blank sample was also included. |
| Sampleprep Protocol Filename: | 4_Sample preparation protocol_UGA_temp_Oct2023_drawdown.docx |
Analysis:
| MB Sample ID: | SA321854 |
| Analysis ID: | AN004851 |
| Analysis Type: | NMR |
| Analysis Protocol File: | 5_Analysis protocol_UGA_temp_Oct2023_drawdown.docx |
| Software Version: | TopSpin version 3.5 |
| Num Factors: | 6 |
| Num Metabolites: | 16 |
| Units: | Intensity |
NMR:
| NMR ID: | NM000268 |
| Analysis ID: | AN004851 |
| Instrument Name: | AVANCE III HD instrument (Bruker) |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 2D-1H-13C |
| Spectrometer Frequency: | 600 MHz |
