Return to study ST002961 main page
MB Sample ID: SA322379
| Local Sample ID: | 20210624_AJU_M3 |
| Subject ID: | SU003074 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003074 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 20210624_AJU_M3 | SA322379 | FL038468 | Control sample | Sample_type |
Collection:
| Collection ID: | CO003067 |
| Collection Summary: | The experiment was initiated by seeding the cells in a 100mm cell culture dish, followed by a 2-day incubation period, during which the culture medium was carefully extracted from the cell culture dish. Use a sterile technique to minimize contamination. Transfer the harvested culture medium to a suitable container. |
| Collection Protocol Filename: | Sample_collection_metabolite.pdf |
| Sample Type: | Cultured fibroblasts |
Treatment:
| Treatment ID: | TR003083 |
| Treatment Summary: | no treatment |
Sample Preparation:
| Sampleprep ID: | SP003080 |
| Sampleprep Summary: | Metabolites were extracted using 80% methanol. In brief, the samples were added with 80% methanol. After vortexing for 1 min and centrifugation at 2000×g for 10 min, supernatant was transferred to a new 1.5 mL tube and completely dried using a HyperVAC-MAX VC2200 centrifugal vacuum concentrator (Hanil Scientific Inc., Korea). Dried metabolite contents were reconstituted in 100 µL of 0.1% formic acid in water and then subjected to LC-MS/MS analysis. |
| Sampleprep Protocol Filename: | Sampleprep_metabolite.pdf |
Combined analysis:
| Analysis ID | AN004862 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity UHPLC |
| Column | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm, 1.8um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Q Exactive™ Hybrid Quadrupole-Orbitrap MS coupled with a 1290 Infinity UHPLC |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003670 |
| Chromatography Summary: | See protocol file, LC_MS_method_metabolite.pdf |
| Methods Filename: | LC_MS_method_metabolite.pdf |
| Instrument Name: | Agilent 1290 Infinity UHPLC |
| Column Name: | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm, 1.8um) |
| Column Temperature: | 320℃ |
| Flow Gradient: | 2.5% solvent B in 5 min, 2.5–12.5% solvent B in 29 min, 12.5–25% solvent B in 11 min, 25–37.5% solvent B in 11 min, 37.5-80% solvent B in 0.1 min, holding at 80% of solvent B in 13.9 min, 80–2.5% solvent B in 0.1 min, 2.5% solvent B for 19.9 min |
| Flow Rate: | 0.2 mL/min |
| Solvent A: | 0.1% formic acid in water |
| Solvent B: | 0.1% formic acid in 80% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004606 |
| Analysis ID: | AN004862 |
| Instrument Name: | Q Exactive™ Hybrid Quadrupole-Orbitrap MS coupled with a 1290 Infinity UHPLC |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Obtained UHPLC-Orbitrap-MS/MS RAW files were processed using Compound Discoverer 3.1.1.12TM (Thermo Fisher Scientific, Waltham, MA, USA). Untargeted metabolomics workflow was used to perform retention time alignment and compound identification. Identification of compounds using mzCloud and ChemSpider |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | MS_analysis_metabolite.pdf |
