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MB Sample ID: SA323872
| Local Sample ID: | 5Cx |
| Subject ID: | SU003098 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | < 5 years old |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003098 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | < 5 years old |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 5Cx | SA323872 | FL038673 | Healthy | Group |
| 5Cx | SA323872 | FL038673 | Nutrilon Pepti | Milk powder |
| 5Cx | SA323872 | FL038673 | T0 | Incubation |
Collection:
| Collection ID: | CO003091 |
| Collection Summary: | In vitro simulation of the gastrointestinal digestion consisted of enzymatic digestion (mouth, stomach, and duodenum) followed by colonic fermentation. ). Samples were taken immediately following the addition of SHIME (Simulator of the Human Intestinal Microbial Ecosystem) medium and fecal inoculum in Brain Heart Infusion (BHI) medium (duodenal samples, T0) and after colonic digestion (T48). |
| Sample Type: | Digestive fluid |
| Collection Method: | Spontaneous defecation |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003107 |
| Treatment Summary: | Two different CMP powder formulations were used, i.e., Nutrilon 1 and Nutrilon Pepti 1 (Nutricia, Amsterdam, The Netherlands). Feces were obtained from 6 children within the original cohort (<2 years old; 50% girls), without a history of antibiotic treatment 6 months prior to donation. Among the 6 participants, 2 children suffered from IgE-CMA, and 2 were designated as healthy controls. In vitro simulation of the gastrointestinal digestion consisted of enzymatic digestion (mouth, stomach, and duodenum) followed by colonic fermentation. |
| Treatment Compound: | Nutrilon 1 and Nutrilon Pepti 1 |
| Treatment Dose: | 920 mg |
| Treatment Doseduration: | 1 |
Sample Preparation:
| Sampleprep ID: | SP003104 |
| Sampleprep Summary: | Internal standard (75 uL of D-valine-d8 and L-alanine-d3 (100 ng/uL) was added to 1.5 mL of digestion fluid, which was then centrifuged (13,300 x g, 5 min). The supernatant was filtered through a polyvinylidene fluoride membrane (0.22 μ m, 33 mm ∅, Millex, USA). The filtrate was then diluted (1/5) with UP water and transferred to a LC-MS vial. An aliquot of 10 μL of sample was injected into the chromatographic system |
| Processing Storage Conditions: | -80℃ |
| Sample Spiking: | Internal standard |
Combined analysis:
| Analysis ID | AN004905 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | a.u. |
Chromatography:
| Chromatography ID: | CH003700 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 45 |
| Flow Gradient: | A gradient profile with following proportions (v/v) of solvent A was applied: 0-1.5 min at 98%, 1.5-7.0 min from 98% to 75%, 7.0-8.0 min from 75% to 40%, 8.0-12.0 min from 40% to 5%, 12.0-14.0 min at 5%, 14.0-14.1 min from 5 to 98%, followed by 4.0 min of re-equilibration. |
| Flow Rate: | 0.4 mL/min |
| Internal Standard: | L-alanine-d3, D-valine-d8 |
| Sample Injection: | 10 uL |
| Solvent A: | 0.1% formic acid in ultrapure water |
| Solvent B: | 0.1% formic acid in acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004648 |
| Analysis ID: | AN004905 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS acquisition: Xcalibur Targeted data processing: Xcalibur Untargeted data acquisition: Compound Discoverer, R studio Ion mode is unspecified as analysis is performed in polarity switching mode, thereby generating data for both positive and negative ionization at the same time. |
| Ion Mode: | UNSPECIFIED |
