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MB Sample ID: SA325676
| Local Sample ID: | 81 |
| Subject ID: | SU003101 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | < 5 years old |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003101 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | < 5 years old |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 81 | SA325676 | FL038732 | 0 | IgE CMA |
| 81 | SA325676 | FL038732 | 0 | IgE other |
| 81 | SA325676 | FL038732 | 0 | non-IgE CMA |
| 81 | SA325676 | FL038732 | 1 | Healthy |
Collection:
| Collection ID: | CO003094 |
| Collection Summary: | Urine samples were collected using The Newcastle Urine Collector (SteriSets Medical Products, Guilhufe, Portugal) or directly in a plastic container. Samples were stored at -20 °C until pick-up (< 1 week). Following cooled transport (dry ice or icepacks). Urine samples were thawed and aliquoted upon arrival and stored at -80 °C awaiting analysis. |
| Sample Type: | Urine |
| Collection Method: | Spontaneous defecation |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003110 |
| Treatment Summary: | No treatment was applied, patients were divided into 4 different control groups: - IgE-mediated cow's milk allergy - other functional gastrointestinal disorder - IgE-mediated other food allergy - healthy brothers and sisters |
Sample Preparation:
| Sampleprep ID: | SP003107 |
| Sampleprep Summary: | Extraction of the urinary metabolome was initiated by pipetting 300 mL urine into a 1.5-mL Eppendorf tube, after which 30 mL of ISTD mixture (100 ng mL&1 D-valine-d8 and of L-alanine-d3) was added. Following this, the sample was centrifuged for 8 min (1 000 x g, 4 %C). Next, 100 mL supernatant was collected and diluted (1:10) with ultrapure water, after which the resulting extract was transferred to a glass HPLC-vial. |
| Processing Storage Conditions: | -80℃ |
| Sample Spiking: | Internal standard |
Combined analysis:
| Analysis ID | AN004908 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | a.u. |
Chromatography:
| Chromatography ID: | CH003703 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 45 |
| Flow Gradient: | A gradient profile with following proportions (v/v) of solvent A was applied: 0-1.5 min at 98%, 1.5-7.0 min from 98% to 75%, 7.0-8.0 min from 75% to 40%, 8.0-12.0 min from 40% to 5%, 12.0-14.0 min at 5%, 14.0-14.1 min from 5 to 98%, followed by 4.0 min of re-equilibration. |
| Flow Rate: | 0.4 mL/min |
| Internal Standard: | L-alanine-d3, D-valine-d8 |
| Sample Injection: | 10 uL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004651 |
| Analysis ID: | AN004908 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS acquisition: Xcalibur Targeted data processing: Xcalibur Untargeted data acquisition: Compound Discoverer, R studio Ion mode is unspecified as analysis is performed in polarity switching mode, thereby generating data for both positive and negative ionization at the same time. |
| Ion Mode: | UNSPECIFIED |
