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MB Sample ID: SA326178
Local Sample ID: | 336ade_norm |
Subject ID: | SU003109 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003109 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
336ade_norm | SA326178 | FL038771 | adenorm | Tissue type |
Collection:
Collection ID: | CO003102 |
Collection Summary: | The patient tissue specimens from 79 NSCLC patients including the two most prevalent histological subtypes adenocarcinoma (ade) and squamous cell (squ) tumor (65 ade patients with age range 36-84; 14 squ patients with age range 49-78) were obtained from Beijing Hospital. Primary tumor tissues, paired adjacent non-cancerous tissues (ANT) (within 2 cm apart from tumor edge) and distant normal tissues (DNT) ( > 2 cm apart from tumor edge) were surgically resected and frozen at −80 °C until metabolite extraction. The study was approved by the Research Ethics Committee of Beijing Hospital (2019BJYYEC-206-02) and also approved by the Ocean University of China (OUC-HM-2022-002). |
Sample Type: | Lung |
Collection Method: | Surgical resect |
Collection Location: | Beijing Hospital |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR003118 |
Treatment Summary: | No. Only cancer and noncancerous tissues. |
Sample Preparation:
Sampleprep ID: | SP003115 |
Sampleprep Summary: | Frozen tissue samples were thawed on ice. Each tissue sample was cut into small pieces and accurately weighed.To 100 mg tissue sample, 1 mL precooled 80% methanol was added and the sample was homogenized (60 Hz, 90 s) in a tissue homogenizer (Jingxin, JX-24, Shanghai, China). After vortexing for 1 min, the mixture was incubated at -80 °C for 4 hours, followed by centrifuging at 14,000 × g for 10 min at 4 °C. The supernatant was transferred to a new tube. To the rest of the sample, 400 μL dichloromethane/ methanol (3:1, v/v) was added for lipid extraction. The sample mixture was then vortexed twice, each time for 30 s, followed by incubation at -80 °C for 30 min. After homogenization (60 Hz, 90 s) and centrifugation of the sample at 14,000 × g for 10 min at 4 °C, the supernatant was collected and dried under a speed vacuum concentrator. |
Combined analysis:
Analysis ID | AN004921 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1290 Infinity |
Column | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo LTQ XL |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH003715 |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
Column Temperature: | 50 |
Flow Gradient: | 0-2 min, 60%-57% A, 2-2.1 min 57%-50% A, 2.1-12 min 50%-46% A, 12-12.1 min 46%-30% A, 12.1-18 min 30%-1% A |
Flow Rate: | 0.3 mL min-1 |
Solvent A: | acetonitrile/water |
Solvent B: | 2-propanol/acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004664 |
Analysis ID: | AN004921 |
Instrument Name: | Thermo LTQ XL |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | capillary temperature 300 °C, capillary voltage 20 V, tube lens 35 V, spray voltage 4.0 kV for positive ion mode, auxiliary gas, sheath gas and sweep gas flow rate 10, 40, and 0 arbitrary units. Progenesis QI version 2.4 was used for raw data processing. FORTRAN based home-built secondary data processing was applied for artefact removal, blank correction, QC -based signal correction and normalization, etc. |
Ion Mode: | POSITIVE |