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MB Sample ID: SA326296
| Local Sample ID: | 222ade_side |
| Subject ID: | SU003109 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
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Subject:
| Subject ID: | SU003109 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 222ade_side | SA326296 | FL038772 | adeside | Tissue type |
Collection:
| Collection ID: | CO003102 |
| Collection Summary: | The patient tissue specimens from 79 NSCLC patients including the two most prevalent histological subtypes adenocarcinoma (ade) and squamous cell (squ) tumor (65 ade patients with age range 36-84; 14 squ patients with age range 49-78) were obtained from Beijing Hospital. Primary tumor tissues, paired adjacent non-cancerous tissues (ANT) (within 2 cm apart from tumor edge) and distant normal tissues (DNT) ( > 2 cm apart from tumor edge) were surgically resected and frozen at −80 °C until metabolite extraction. The study was approved by the Research Ethics Committee of Beijing Hospital (2019BJYYEC-206-02) and also approved by the Ocean University of China (OUC-HM-2022-002). |
| Sample Type: | Lung |
| Collection Method: | Surgical resect |
| Collection Location: | Beijing Hospital |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003118 |
| Treatment Summary: | No. Only cancer and noncancerous tissues. |
Sample Preparation:
| Sampleprep ID: | SP003115 |
| Sampleprep Summary: | Frozen tissue samples were thawed on ice. Each tissue sample was cut into small pieces and accurately weighed.To 100 mg tissue sample, 1 mL precooled 80% methanol was added and the sample was homogenized (60 Hz, 90 s) in a tissue homogenizer (Jingxin, JX-24, Shanghai, China). After vortexing for 1 min, the mixture was incubated at -80 °C for 4 hours, followed by centrifuging at 14,000 × g for 10 min at 4 °C. The supernatant was transferred to a new tube. To the rest of the sample, 400 μL dichloromethane/ methanol (3:1, v/v) was added for lipid extraction. The sample mixture was then vortexed twice, each time for 30 s, followed by incubation at -80 °C for 30 min. After homogenization (60 Hz, 90 s) and centrifugation of the sample at 14,000 × g for 10 min at 4 °C, the supernatant was collected and dried under a speed vacuum concentrator. |
Combined analysis:
| Analysis ID | AN004921 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity |
| Column | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo LTQ XL |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003715 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0-2 min, 60%-57% A, 2-2.1 min 57%-50% A, 2.1-12 min 50%-46% A, 12-12.1 min 46%-30% A, 12.1-18 min 30%-1% A |
| Flow Rate: | 0.3 mL min-1 |
| Solvent A: | acetonitrile/water |
| Solvent B: | 2-propanol/acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004664 |
| Analysis ID: | AN004921 |
| Instrument Name: | Thermo LTQ XL |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | capillary temperature 300 °C, capillary voltage 20 V, tube lens 35 V, spray voltage 4.0 kV for positive ion mode, auxiliary gas, sheath gas and sweep gas flow rate 10, 40, and 0 arbitrary units. Progenesis QI version 2.4 was used for raw data processing. FORTRAN based home-built secondary data processing was applied for artefact removal, blank correction, QC -based signal correction and normalization, etc. |
| Ion Mode: | POSITIVE |
