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MB Sample ID: SA327488
| Local Sample ID: | 20220725.82.fid#82 |
| Subject ID: | SU003129 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003129 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 20220725.82.fid#82 | SA327488 | FL038862 | Normal tissue | Fator |
Collection:
| Collection ID: | CO003122 |
| Collection Summary: | Tissue samples, including tumor and normal areas 5 cm away, were obtained under the guidance of experienced pathologists without compromising the patients' pathology examinations. The collected tissue was rinsed with PBS to avoid contamination, excess moisture was removed, and it was rapidly frozen in liquid nitrogen to arrest enzymatic or chemical reactions. Samples were stored at −80°C until metabolite extraction. |
| Collection Protocol Filename: | Tissue_collection_uploaded.pdf |
| Sample Type: | Tissue |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003138 |
| Treatment Summary: | None |
Sample Preparation:
| Sampleprep ID: | SP003135 |
| Sampleprep Summary: | Tissue homogenate preparation: Tissue samples weighing 300 mg were ground using a 60 Hz grinder at 4 °C for 1 min in a mixture of 4 mL/g of CH3OH and 2 mL/g of ultrapure water. The resulting homogenate was then subjected to vortexing for 1 min after adding 4 mL/g of CHCl3 and 4 mL/g of ultrapure water. The mixture was allowed to settle on ice for 15 mins and subsequently centrifuged at 10,000 rpm for 10 mins at 4 °C. The supernatant was carefully transferred to a new 5 mL Eppendorf (EP) tube and treated with running nitrogen to remove the methanol. The resulting liquid was freeze-dried at −80°C until further analysis. The freeze-dried powder was dissolved in 550 μL of PBS/D2O buffer (pH 7.4, 150 mM), which contained 0.05% TMSP-2,2,3,3-D4 (D, 98%) SODIUM-3-TRIMETHYLSILYLPROPIONATE (TSP, Cambridge Isotope Laboratories (CIL), Inc. #DLM-48, CAS #24493-21-8)). After thorough mixing, the solution was centrifuged at 10,000 rpm for 10 min at 4 °C. Finally, 500 μL of the supernatant was transferred into a 5 mm NMR tube (NORELL, #S55 SECURE SERIES) for analysis. |
| Sampleprep Protocol Filename: | Tissue_preparation.pdf |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | 4℃ |
Analysis:
| MB Sample ID: | SA327488 |
| Analysis ID: | AN004948 |
| Analysis Type: | NMR |
| Results File: | ST003015_AN004948_Results.txt |
| Units: | gauss |
NMR:
| NMR ID: | NM000273 |
| Analysis ID: | AN004948 |
| Instrument Name: | 600 MHz Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 600 MHz |
| NMR Solvent: | H2O+D2O |
